PURPOSE: To investigate the isolating and cultuiing method of nanobacteria from dental pulp stone ami make the identification of nanobacleria. METHODS: Freshly collected 27 dental pulp stones were divided into nine samples. Each sample contained three dental pulp stones. All samples were used for the isolation and culture of nanobacteria. The shape and the growth characteristics of the cultured bacteria were observed. Nanobacteria were identified by von kossa staining, immunohistochemical staining, indirect...