【摘要】 土壤-植物系统是土壤生态系统中与人类生存和健康最为密切的系统之一.为研究生物炭对抗生素污染土壤-植物系统中微生物群落的影响，通过设置抗生素及生物炭添加下为期60 d的土壤-植物盆栽实验，基于高通量测序技术分析土壤及植物在不同处理组中细菌群落微生物的组成变化.结果表明，抗生素添加后显著降低了土壤类诺卡氏菌属(Nocardioides)细菌群落的相对丰度，增加了植物鞘脂单胞菌科(Sphingomonadaceae)细菌群落的相对丰度.生物炭的添加增加了土壤酸杆菌门(Acidobacteria)的丰度，但对植物微生物群落的影响不显著.综上，添加低质量分数的抗生素能够刺激土壤-植物系统中的微生物群落，在一定程度上提高了细菌群落的丰度，同时也增强了微生物群落之间的稳固性.而生物炭的添加对微生物群落的影响不显著，这与生物炭质量分数较低有关.本研究有助于加深生物炭对抗生素污染背景下土壤-植物系统中微生物群落变化的理解.更多还原

【Abstract】 One of the soil ecosystem’s most intimate relationships between human life and wellness is the soil-plant system. A soil-plant pot experiment with antibiotics and biochar addition was carried out for 60 d in order to study the effects of biochar on the microbial community in soil-plant systems repaired by antibiotics. Based on high-throughput sequencing technology, the changes in the microbial community composition of soil and plants in different treatment groups were analyzed. The findings demonstrate that the relative abundance of Sphingomonadaceae is enhanced and the relative abundance of Nocardioides is dramatically lowered by antibiotic administration. The plant microbial community is not significantly affected by the addition of biochar, however the amount of Acidobacteria in the soil is enhanced. In summary, low amounts of antibiotics can be added to soil-plant systems to stimulate microbial communities, increase bacterial community abundance, and improve microbial community stability. However, because of the low concentration of biochar, the addition of biochar has no discernible impact on the microbial community. In the context of antibiotic contamination, this study contributes to a deep knowledge of the effects of biochar on alterations in the microbial population in the soil-plant system.更多还原