【摘要】 本文以梵净山石斛、矩唇石斛、喉红石斛、翅萼石斛、铁皮石斛和金钗石斛的杂交石斛种子为研究对象，对梵净山石斛（♀）×矩唇石斛（♂）、喉红石斛（♀）×翅萼石斛（♂）、铁皮石斛（♀）×金钗石斛（♂）3组石斛进行人工授粉种间杂交，对所得杂交石斛种子开展种子无菌萌发、杂交石斛种苗壮苗生根以及移栽驯化研究。结果表明：最适合3种杂交石斛种子无菌萌发的基本培养基均为1/2MS培养基；最佳壮苗生根培养基配方均为1/2MS+BA 0.1 mg·L^-1+NAA 0.5 mg·L^-1+100 g·L^-1马铃薯+0.5 g·L^-1活性炭；适宜移栽驯化的基质分别为优品兰土、精品兰土、优品兰土，成活率分别达到90.6%、62.5%、100%。通过人工授粉得到种子，以杂交石斛种子为外植体，可以建立杂交石斛快繁技术体系。更多还原

【Abstract】 Taking the hybrid seeds of Dendrobium fanjingshanense, D.linawianum, D.christyanum, D.cariniferum, D.offi cinale and D.nobile as research objects, this study aims to explore the sterile sowing and rapid propagation.Three groups were also involved for the artificial pollination: D.fanjingshanense（♀） × D.linawianum（♂）, D.christyanum（♀） × D.cariniferum（♂）, and D.officinale（♀） × D.nobile（♂）.The hybrid Dendrobium seeds were then used to evaluate the sterile germination, the rooting of hybrid Dendrobium seedlings, as well as transplanting and domestication.The results showed that the 1/2MS medium was the most suitable basal medium for the aseptic germination of three species of Dendrobium orchids.The optimal composition of medium was 1/2MS+BA 0.1 mg·L^-1+NAA 0.5 mg·L^-1+potato 100 g·L^-1+ activated carbon 0.5 g·L^-1 for the strong seedlings and rooting.The Youpin, Fine and Youpin orchid soil substrates were more suitable for transplanting and growing, where the survival rates were 90.6%, 62.5%, and 100%, respectively.The seeds were obtained after artificial pollination.Taking the hybrid seeds as the explants, the efficient and rapid propagation can be expected to establish for the tissue culture.更多还原