【摘要】 针对生物法制备苯乳酸的分离纯化问题，利用多微管反应器制备纳米纤维素嵌合型晶胶微球，并接枝N,N-二甲基氨乙基甲基丙烯酰胺，用于苯乳酸的层析分离。结果表明：晶胶微球的粒径在4～6 mm，有效孔隙率达70%以上，绝干孔隙率为87%～91%；以牛血清白蛋白考察3种不同纤维素含量（质量分数为1%、3%、5%）微球填充柱的吸附性能，吸附容量分别为1.72、2.84、4.49 mg·m L^-1。对苯乳酸的吸附条件进行优化表明，上样液pH为6时苯乳酸吸附容量最大，为41.32 mg·m L^-1；上样流速增大，苯乳酸吸附容量有所下降；对填充柱进行连续5次吸附解吸附苯乳酸，得到吸附容量在24.17～26.31 mg·m L^-1。研究结果可为苯乳酸的分离材料选用提供参考。更多还原

【Abstract】 Cryogel beads embedded with nanocellulose were prepared using a multi-micro-tube reactor,which were grafted with N,N-dimethylaminoethyl methacrylamide and used as the chromatographic adsorbents in the bioproduction of phenyllactic acid.The results show that these cryogel beads have particle sizes of 4 to 6 mm,over 70%effective porosity and 87%to 91%absolute porosity.The adsorption properties of cryogel beads embedded with different contents of cellulose （mass fractions of 1%,3%and 5%,respectively） packed in columns were investigated for bovine serum albumin adsorption,and the adsorption capacities were 1.72,2.84and 4.49 mg·m L^-1,respectively.The phenyllactic acid adsorption conditions were optimized experimentally.The results show that the adsorption capacity decreases with the increase of sample flow rate and the maximum adsorption capacity is 41.32 mg·m L^-1 at the sample solution pH of 6.Five runs of chromatographic adsorption and desorption of phenyllactic acid were repeated in the packed column and the adsorption capacity were between 24.17 and 26.31 mg·m L^-1,which indicates that the cryogel beads have potential for phenyllactic acid separation in bioproduction更多还原