【摘要】 目的 探讨痛风性关节炎(GA)患者血清抵抗素(RETN)、Beclin-1的表达特点，分析其与GA临床特征及临床疗效的关系。方法 将2019年1月至2022年12月东莞市人民医院收治的82例GA患者(GA组)和60例健康志愿者(对照组)纳入研究。GA患者治疗前后(对照组体检当日)检测血清RETN、Beclin-1的表达，比较不同临床特征和疗效GA患者血清RETN、Beclin-1表达差异。Pearson相关性分析GA患者血清RETN、Beclin-1表达与尿酸(UA)水平的相关性。采用受试者工作特征(ROC)曲线分析血清RETN、Beclin-1对GA的诊断价值。结果 GA组血清RETN水平高于对照组，Beclin-1相对表达水平低于对照组(P<0.05)。急性期、病程≥5年、受累关节≥5个、年发作频率≥3次GA患者血清RETN水平高于慢性期和间歇期、病程<5年、受累关节<5个、年发作频率<3次GA患者(P<0.05),Beclin-1相对表达水平低于慢性期和间歇期、病程<5年、受累关节<5个、年发作频率<3次GA患者(P<0.05)。GA患者血清UA水平与RETN水平呈正相关(r=0.674,P<0.05),与Beclin-1相对表达水平呈负相关(r=-0.568,P<0.05)。有效组治疗后血清RETN水平低于无效组，Beclin-1相对表达水平高于无效组(P<0.05)。联合RETN、Beclin-1诊断GA的曲线下面积为0.921,高于单独诊断(Z=3.752、3.154,P<0.05)。结论 GA患者血清RETN水平增高，Beclin-1相对表达水平降低，且与UA水平增高、GA急性期、病程延长、受累关节数量和年发作频率增加及治疗无效有关。RETN、Beclin-1可作为GA诊断的标志物。更多还原

【Abstract】 Objective To investigate the expression characteristics of serum resistin(RETN) and Beclin-1 in patients with gouty arthritis(GA),and to analyze the relationship between RETN and GA clinical characteristics and clinical efficacy.Methods A total of 82 GA patients(GA group) and 60 healthy volunteers(control group) in Dongguan People′s Hospital from January 2019 to December 2022 were enrolled in the study.The expressions of serum RETN and Beclin-1 in GA patients were detected before and after treatment(on the physical examination day of the control group),and the differences of serum RETN and Beclin-1 in GA patients with different clinical characteristics and efficacy were compared.Pearson correlation analysis was used to investigate the correlation between serum RETN,Beclin-1 expression and uric acid(UA) level in GA patients.The diagnostic value of serum RETN and Beclin-1 in GA was analyzed using the receiver operating characteristic(ROC) curve.Results The serum RETN level in the GA group was higher than that in the control group, and the expression of Beclin-1 was lower than that in the control group(P<0.05).The serum RETN levels in GA patients with acute stage, disease duration ≥5 years, affected joints ≥5,annual attack frequency ≥3 times were higher than those in GA patients with chronic stage and intermittent stage, disease duration <5 years, affected joints <5,annual attack frequency <3 times(P<0.05),and the expression of Beclin-1 were lower than those in GA patients in chronic and intermittent stages, disease duration <5 years, affected joints <5,annual frequency <3 times(P<0.05).The serum UA level in GA patients was positively correlated with RETN(r=0.674,P<0.05),and negatively correlated with Beclin-1 expression(r=-0.568,P<0.05).After treatment, the serum expression of level RETN in the effective group was lower than that in the ineffective group, while the level Beclin-1 was higher than that in the ineffective group(P<0.05).The area under the curve of combined RETN and Beclin-1 diagnosis for GA was 0.921,which was higher than that of individual diagnosis(Z=3.752,3.154,P<0.05).Conclusion Serum RETN level increases, and Beclin-1 expression decreases in GA patients, which is associated with increased UA level, prolonged acute stage and course of GA,increased number of affected joints and annual attack frequency, and treatment ineffectiveness.RETN and Beclin-1 could serve as biomarkers for GA diagnosis.更多还原