【摘要】 为提升葡萄糖溶液的检测性能，首先对葡萄糖溶液中体积分数为99%的吡喃葡萄糖进行仿真计算，获得理论拉曼光谱；其次，使用拉曼光谱系统获取不同质量浓度葡萄糖溶液的实验拉曼光谱，理论计算结果显示，两种吡喃葡萄糖在1125 cm-1处均有特征峰，与实验结果一致；再次，选择水的1640 cm-1特征峰作为内标峰进行归一化；最后，采用特征峰相对强度和特征峰相对面积两种定量分析方法进行线性分析，线性相关系数分别为0.992和0.985，最低检测限为48.1 mg/dL和52.3 mg/dL，最低检测质量浓度均为12.5 mg/dL。结果表明，相对强度法简单方便，线性拟合度更高，更适用于低质量浓度葡萄糖溶液的精确定量分析。更多还原

【Abstract】 To improve the detection performance of glucose solutions, we first simulate and calculate the theoretical Raman spectrum of 99%(volume fraction) glucopyranose in the glucose solution. Next, we obtain experimental Raman spectra of glucose solutions at different mass concentrations using a Raman spectroscopy system. The theoretical calculations show that both types of glucopyranose exhibit characteristic peaks at 1125 cm-1, which is consistent with the experimental results. We then select the 1640 cm-1 characteristic peak of water as the internal standard peak for normalization. Finally, two quantitative analysis methods, the characteristic peak relative intensity method and the characteristic peak relative area method, are used for linear analysis. The linear correlation coefficient are 0.992 and 0.985,respectively. The lowest detection limits are 48.1 mg/dL and 52.3 mg/dL, and the lowest detection mass concentrations are all 12.5 mg/dL. The results indicate that the relative intensity method is simple and convenient, and provides a higher linear fitting, making it more suitable for accurate quantitative analysis of low mass concentration glucose solutions.更多还原