【摘要】 采用超高效液相色谱-串联质谱法(UPLC-MS/MS)测定氨甲苯酸中乌洛托品残留含量的方法。采用Thermo Syncronis HILIC(100×2.1,1.7μm)色谱柱，流动相为乙腈-乙酸铵甲酸水溶液，流速0.3 mL/min,柱温：30℃,进样量10μL,定量离子对为141.1/112.1,检测模式为多重反应监测(MRM)。乌洛托品在10～1000 ppb(R~2=0.9992)范围内线性良好，方法的检出限为3ng/mL,加标回收率为89%～103%。该方法简便、准确、灵敏度高，能够满足氨甲苯酸中乌洛托品残留的检测。更多还原

【Abstract】 Hexamethylenetetramine residues in aminomethylbenzoic acid by UPLC-MS/MS was determined. The chromatographic column was Thermo Syncronis HILIC(100×2.1,1.7 μm). The mobile phase was acetonitrile-10 mmol/L ammonium acetate aqueous solution. The flow rate was 0.3 mL/min and the column temperature was 30 ℃. The injection volume is 10μL. The quantitative ion pair was 141.1/112.1 and the detection mode was multiple reaction monitoring(MRM). Hexamethylenetetramine had a good linearity in the range of 10～1 000 ppb(R~2=0.9992),and the detection limit of the method was 3 ng/mL. Moreover, the recovery rates range from 89% to 103% at different concentration levels. A simple, accuracy and high sensitivity method is provided for the determination of hexamethylenetetramine content.更多还原