【摘要】 目的 观察红花逍遥片对卵巢早衰（POF）模型大鼠的影响。方法 20只大鼠随机分成正常组（a组）、模型组（b组）、红花逍遥片组（c组）、坤泰胶囊组（d组）,每组5只。通过阴道脱落细胞涂片观察大鼠动情周期，采用酶联免疫吸附技术（ELISA）检测大鼠血清激素水平、RT-PCR法检测LC3、Bcl-2、Beclin-1、ATG13、MDA、ROS mRNA水平。结果 与a组比较，b组FSH、LH水平明显升高（P<0.05）,d组LH水平升高（P<0.05）,b、c、d组E₂、AMH水平明显降低（P<0.05）,ATG13、MDA、ROS明显升高（P<0.05）;b组、d组LC3、Beclin-1明显升高（P<0.05）,b组Bcl-2明显升高（P<0.05）;与b组比较，c组、d组FSH、LH、LC3、Bcl-2、Beclin-1、ATG13、ROS水平明显降低（P<0.05）,E₂、AMH水平明显升高（P<0.05）;d组MDA明显降低（P<0.05）。c组与d组各指标水平比较，差异无统计学意义（P>0.05）。结论 红花逍遥片能够改善POF模型大鼠氧化应激状态及自噬水平，提高POF模型大鼠卵巢功能。更多还原

【Abstract】 Objective To observe the effects of Honghua Xiaoyao tablet on premature ovarian failure（POF） model rats. Methods Twenty rats were randomly divided into normal group（group a）, model group（group b）, Honghua Xiaoyao group（group c） and Kuntai capsule group（group d）, with 5 rats in each group. The estrus cycle of rats was observed by vaginal smear, the serum hormone levels were detected by enzyme-linked immunosorbent assay（ELISA）, and the levels of LC3, Bcl-2, Beclin-1, ATG13, MDA and ROS mRNA were detected by RT-PCR. Results Compared with group a, FSH and LH levels in group b were significantly increased（P<0.05）, LH levels in group d were significantly increased（P<0.05）, E₂ and AMH levels in groups b, c and d were significantly decreased（P<0.05）, and ATG13, MDA and ROS were significantly increased（P<0.05）. LC3 and Beclin-1 in group b and d were significantly increased（P<0.05）, and Bcl-2 in group b was significantly increased（P<0.05）. Compared with group b, the levels of FSH, LH, LC3, Bcl-2, Beclin-1, ATG13 and ROS in groups c and d were significantly decreased（P<0.05）, while the levels of E₂ and AMH were significantly increased（P<0.05）. MDA was significantly decreased in group d（P>0.05）. Conclusion Honghua Xiaoyao tablets can improve the oxidative stress state and autophagy level of POF model rats, and improve the ovarian function of POF model rats.更多还原