【摘要】 目的 分析成都口岸输入性新冠病毒全基因组序列，掌握成都口岸输入性新冠病毒变异株的分布情况。方法 随机选取2022年10月—2023年3月成都口岸58例新冠病毒感染者的鼻咽拭子样本，采用第三代Nanopore纳米孔测序平台进行新冠病毒全基因组测序，应用Baiyi全基因组分析软件系统判定Pangolin分型及覆盖度，分析输入性新冠病毒变异株分布情况。结果 测序共获得58条新冠病毒全基因组序列，其中序列覆盖度大于99%的53例，Pangolin分型均为Omicron变异株。数量最多的5种变异株依次是BA.5 (21株，36.21%),XBB.1 (9株，15.52%),BQ.1(7株，12.07%),BF.7(5株，8.62%),BA.2(3株，5.17%)，与全球主导流行株基本一致。结论 成都口岸境外输入性新冠病毒变异株与全球的流行趋势基本一致。更多还原

【Abstract】 Objective To analyze the whole genome sequence of imported severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) isolated at Chengdu port,and to understand the distribution of imported SARS-CoV-2 variants at Chengdu port. Methods Nasal and pharyngeal swab samples of 58 SARS-CoV-2 infected patients at Chengdu port from October 2022 to March 2023 were randomly selected,whole genome of SARS-CoV-2 was sequenced using the third-generation Nanopore sequencing platform,and the classification and coverage of Pangolin was determined using Baiyi whole genome analysis software system. The distribution of imported SARS-CoV-2 variants was analyzed. Results A total of 58 whole genome sequences of SARS-CoV-2 were obtained,of which 53 cases with sequence coverage greater than 99% were Omicron variants. The five most numerous variants were BA.5(21 strains,36.21%),XBB.1(9strains,15.52%),BQ.1(7 strains,12.07%),BF.7(5 strains,8.62%),BA.2(3 strains,5.17%),which were consistent with the dominant strains in the world. Conclusion The imported SARS-CoV-2 variants distribution at Chengdu port was consistent with the global epidemic trend.更多还原