【摘要】 目的 分析河北省本土新冠病毒感染（COVID-19）疫情病例新冠病毒（SARS-CoV-2）全基因组特征，结合流行病学开展溯源调查，为疫情的精准防控提供技术支撑。方法 对河北省2021年10-11月发生的一起本土疫情的部分COVID-19确诊病例，采集咽拭子标本，利用高通量测序技术开展病毒全基因组测序，分析病毒核苷酸和氨基酸序列变异特征。结果 共获得本起疫情相关的68条高质量病毒全基因组序列，均为Delta变异株AY.126进化分支。经比对，首发病例与同期内蒙古额济纳旗疫情病毒序列高度同源，仅有两个碱基存在差异，68条序列与武汉参考株（NC＿045512）相比，核苷酸突变总数有44～48个，核苷酸突变类型以T转换为主；在S蛋白上发现D614G、L452R、T478K、P681R等10个氨基酸变异及2个氨基酸缺失。结论 对疫情早期病例、关键病例开展SARS-CoV-2全基因组测序，能指明流行病学调查方向，跟踪病毒基因变异特征，为疫情防控提供有力技术支撑。更多还原

【Abstract】 Objective To analyze the whole genome characteristics of SARS-CoV-2 in local COVID-19 epidemic,and to carry out traceability investigation based on epidemiology,so as to provide technical support for the accurate prevention and control of the epidemic. Methods The throat swab samples were collected from some confirmed cases of COVID-19 in a local epidemic in Hebei Province from October to November 2021,the whole genome of the viruses were sequenced by high-throughput sequencing technology to analyze the variation characteristics of viral nucleotide and amino acid sequences. Results A total of 68 high-quality virus genome sequences related to this epidemic were obtained,all of which were the evolutionary branch of Delta variant AY.126. After comparison,the first case sequence was highly homologous with the virus sequence of the Ejina Banner epidemic in Inner Mongolia during the same period,and the total number of nucleotide mutations in 68 sequences was 44-48 compared with Wuhan reference strain(NC＿045512),the nucleotide mutation type was converted to primary by T. On S protein,10amino acid mutations such as D614G,L452R,T478K,P681R and 2 amino acid deletions were found. Conclusion SARSCoV-2 whole genome sequencing for early cases and key cases could quickly indicate the direction of epidemiological investigation,and tracked the characteristics of virus gene variation,which could provide strong technical support for epidemic prevention and control.更多还原