Objective To develop a model of traumatic injury with secondary hypoxia insult on cultured rats astrocytes in vitro.Methods Rat cortical astrocytes cultured in tissue culture wells with a flexible silastic bottom were injured by stretch device.The pressure of the stretch device can be controlled and on line tested by the computer.After stretch-induced injury,the astrocytes were insulted by 1h hypoxia stress.Extent of cell injury was qualitatively assessed by lactate dehydrogenase(LDH) enzyme release and...