【摘要】 为明确萎缩芽孢杆菌Bacillus atrophaeus XW-2对松材线虫Bursaphelenchus xylophilus的杀线活性,开发松材线虫病生防制剂,研究通过室内浸渍试验,测定了XW-2菌悬液和发酵上清液对松材线虫的杀线活性。结果表明, XW-2的菌悬液和发酵上清液原液处理松材线虫12 h时,松材线虫死亡率分别为100%和91.00%;处理24 h的死亡率均可达100%;随着稀释倍数的增大,线虫死亡率逐渐降低,稀释100倍时,菌悬液处理松材线虫24 h的线虫死亡率为86.67%,发酵上清液处理的线虫死亡率为63.00%。因此, XW-2菌悬液和发酵上清液对松材线虫均具有杀线活性,相同浓度下的菌悬液杀线活性显著(P<0.05)高于发酵上清液。更多还原

【Abstract】 To determine the killing activity of Bacillus atrophaeus XW-2 against the Bursaphelenchus xylophilus,a biocontrol agent against pine wood nematode disease was developed. The killing activity of XW-2bacterial suspension and fermentation supernatant against Bursaphelenchus xylophilus was determined by the indoor immersion test. The results showed that when XW-2 bacterial suspension and fermentation supernatant were used to treat pine wood nematodes for 12 hours,the correction rates for pine wood nematodes were 100%and 91. 00%,respectively. However,after 24 h of treatment,the corrected mortality rates reached 100%. As the dilution concentration increases,the mortality rate of nematodes gradually decreases. However,after diluting the bacterial suspension 100 times and treating nematodes for 24 hours,the corrected mortality rate of nematodes is 86. 67%. However,after diluting the fermentation supernatant 100 times and treating pine wood nematodes for 24 hours,the corrected mortality rate of nematodes is 63. 00%. Therefore,the XW-2 bacterial suspension and fermentation supernatant both had bactericidal activity against pine wood nematodes,and the bactericidal activity of the bacterial suspension at the same concentration was significantly(P<0. 05) higher than that of the fermentation supernatant.更多还原