【摘要】 为了探究丹霞梧桐Firmiana danxiaensis不同器官的代谢物差异及药用价值,采用气相色谱—质谱联用技术(Gas chromatography-mass spectrometry, GC-MS)对其根、茎和叶进行代谢物分析与鉴定,利用主成分分析(Principal component analysis, PCA)、正交偏最小二乘法判别分析(Orthogonal partial least squares discriminant analysis, OPLS-DA)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes, KEGG)通路富集分析对不同器官的代谢物进行筛选与富集分析。结果显示,共检测到10 558个代谢物,包括5 184个差异代谢物,其中根和茎的差异代谢物2 965个,根和叶的差异代谢物3 611个,茎和叶的差异代谢物3 662个。KEGG分析结果显示,差异代谢物在黄酮和类黄酮的生物合成通路中富集较为明显,丹霞梧桐的核心差异代谢物中共有16个黄酮化合物参与代谢。更多还原

【Abstract】 To explore the differences and medicinal value of metabolites in different organs of Firmiana danxiaensis,the metabolites extracted from root,stem and leaves were analyzed and identified by Gas chromatography-mass spectrometry(GC-MS). Principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA),and Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment analysis were used to screen and enrich metabolites in different organs. The results showed that 10 558 metabolites were detected,including 5 184 differential metabolites. Among them,there were 2 965 differential metabolites between root and stem, 3 611 differential metabolites between root and leaf,and 3 662 differential metabolites between stem and leaf. The results of the KEGG pathway analysis showed that the differential metabolites were significantly enriched in the biosynthetic pathways of flavonoids and flavonols. A total of 16 flavonoids were found to be involved in the metabolism of the core differential metabolites of F. danxiaensis.更多还原