【摘要】 目的:探讨miRNA-10a对肝硬化大鼠肝切除后肝再生的影响。方法:将36只肝硬化大鼠随机分为假手术组(A组)、70%肝切除未转染组(B组)、70%肝切除转染组(C组),每组12只。术前2周成功将miRNA-10a肝向性转染C组大鼠;于术后12、24、48、72 h每组各处死3只大鼠,取腹主动脉血检测各组大鼠血清谷丙转氨酶(ALT)、白蛋白(ALB)的变化,PCR法检测肝组织中miRNA-10a的水平,计算不同时间大鼠的肝体积比。结果:术后12、24、48、72 h时C组大鼠肝组织中miRNA-10a水平显著高于其他两组,表明已经成功过表达了miRNA-10a;术后12、24、48、72 h时C组大鼠的血清ALT水平均低于B组,ALB水平均高于B组,表明miRNA-10a有利于保护肝功能;术后48、72 h时,C组大鼠的肝体积比显著高于B组,表明过表达miRNA-10a有利于肝组织生长。结论:过表达miRNA-10a能促进肝硬化大鼠肝切除后的肝再生。更多还原

【Abstract】 Objective: To investigate the effect of miRNA-10a on liver regeneration in rats after liver resection in liver cirrhosis.Methods: Thirty-six cirrhotic rats were randomly divided into a sham operation group(Group A, n=12), a 70% liver resection without transfection group(Group B, n=12), and a 70% liver resection with transfection group(Group C, n=12).Two weeks before surgery, successful hepatocyte-specific transfection of miRNA-10a was performed in rats of Group C. At 12h, 24h, 48h, and 72h after surgery, 3 rats from each group were euthanized, and blood samples from the portal vein were collected for testing serum liver function indicators including alanine aminotransferase(ALT) and albumin(ALB). The levels of miRNA-10a in liver tissue were detected using PCR, and the liver-to-body weight ratio was calculated at different time points.Results: At 12 h, 24 h, 48 h, and 72 h after surgery, the levels of miRNA-10a in liver tissue of Group C rats were significantly higher than the other two groups, indicating successful overexpression of miRNA-10a. At 12 h, 24 h, 48 h,and 72 h after surgery, the serum ALT levels of Group C rats were lower on average than Group B, while the ALB levels were higher on average than Group B, suggesting that miRNA-10a is beneficial for protecting liver function. At 48 h and 72 h after surgery, the liver-to-body weight ratio of Group C rats was significantly higher than Group B, indicating that overexpression of miRNA-10a promotes liver tissue growth.Conclusion: Overexpression of miRNA-10a promotes liver regeneration in rats with liver cirrhosis after hepatectomy.更多还原