【摘要】 棕色海藻的一种天然提取物岩藻黄质，近年来受到科学研究的关注，尤其在抗疲劳领域。作为一种具有高度抗氧化活性的物质，已被证明能够显著降低运动后的氧化损伤，并有效缓解疲劳。本研究选用大鼠并分为六组，包括对照组、EIF模型组、不同剂量的岩藻黄质处理组以及岩藻黄质和Nrf2抑制剂ML385的联合处理组。经过4周的实验后，结果发现与EIF模型组相比，岩藻黄质处理组的力竭游泳时间明显延长，同时血清中LDH、CK、cTnI的水平降低，骨骼肌中的抗氧化酶如SOD、CAT、GSH-Px活性增强，而MDA水平下降。此外，岩藻黄质处理增强了Nrf2及其下游靶基因HO-1和NQO-1的表达，而抑制了Keap1的表达。然而，当使用Nrf2抑制剂ML385时，上述岩藻黄质带来的抗EIF效果和对Nrf2-ARE通路的激活都被抑制。综上，岩藻黄质通过激活Nrf2-ARE通路有效缓解EIF，展现出作为一种高效抗EIF保健成分的潜力。显然，岩藻黄质有望成为未来抗疲劳领域的重要天然成分，并有潜力在运动补充剂或食品添加剂中得到广泛应用。更多还原

【Abstract】 Fucoxanthin, a natural extract from brown seaweed, has garnered scientific attention in recent years,especially in the anti-fatigue domain. As a substance with potent antioxidant properties, it has been proven to significantly reduce oxidative damage post-exercise and effectively mitigate fatigue. In this study, rats were divided into six groups, including a control group, an EIF model group, different dosages of fucoxanthin treatment groups, and a combined treatment group of fucoxanthin and Nrf2 inhibitor ML385. After a 4-week experiment,results showed that compared to the EIF model group, the fucoxanthin-treated groups significantly extended their exhaustive swimming time. Concurrently, the serum levels of LDH, CK, and cTnI decreased, while the antioxidative enzymes in skeletal muscle such as SOD, CAT, and GSH-Px increased in activity and MDA levels dropped.Moreover, fucoxanthin treatment upregulated the expression of Nrf2 and its downstream target genes HO-1 and NQO-1, while suppressing Keap1 expression. However, when the Nrf2 inhibitor ML385 was applied, the anti-EIF effects and activation of the Nrf2-ARE pathway brought by fucoxanthin were inhibited. In conclusion, fucoxanthin effectively alleviates EIF through the activation of the Nrf2-ARE pathway, showing its potential as a highly effective anti-EIF health component. Evidentl y, fucoxanthin holds promise as a crucial natural ingredient in the future anti-fatigue domain, with potential widespread applications in sports supplements or food additives.更多还原