【摘要】 为分析干旱胁迫下白菜型油菜基因的表达情况，本研究以登木油菜为试验材料，苗期正常灌溉为对照，干旱8 d为处理组。通过分析登木油菜不同水分条件下差异表达的基因，发现在不同水分条件下显著性差异基因共有3 057个，其中显著性差异上调基因1 616个，显著性差异下调基因1 441个。GO注释的显著差异上调基因共7 172个，显著差异下调基因共731个；通过KEGG通路富集分析，发现参与干旱胁迫的上调差异基因富集的通路主要有RNA降解、真核生物核糖体的生物合成、核糖体、嘧啶代谢、嘌呤代谢等，参与干旱胁迫的下调差异基因富集的通路主要有缬氨酸、亮氨酸和异亮氨酸降解、植物激素信号转导、光合作用、亚油酸、α-亚麻酸代谢等；发现转换SNP位点共285 555个，颠换SNP位点共485 985个。本研究结果表明干旱胁迫下登木油菜基因表达有差异，丰富了白菜型油菜的基因信息资源库，为后期分子育种奠定基础。更多还原

【Abstract】 In order to explore the gene expression of Brassica campestris L. under drought stress, Dengmu rape was used as the test material, normal irrigation at seedling stage as the control, and drought for 8 days as the treatment group. By analyzing the differentially expressed genes under different water conditions in Dengmu rape, we found that there were 3 057 significantly different genes under different water conditions, including 1 616 significantly up-regulated genes and 1 441 significantly down regulated genes. There were 7 172 up-regulated genes and 731 down regulated genes in GO annotation; through the enrichment analysis of KEGG pathway, we found that RNA degradation, eukaryotic ribosome biosynthesis, ribosome, pyrimidine metabolism and purine metabolism were the main pathways involved in the enrichment of up-regulated differential genes under drought stress. The main pathways involved in the down-regulation of differential gene enrichment under drought stress were valine, leucine and isoleucine degradation, plant hormone signal transduction, photosynthesis, linoleic acid, α-linolenic acid metabolism, etc.; a total of 285 555 transformed SNPs and 485 985 transversed SNPs were found. These results indicated that there were differences in gene expression of Dengmu rape under drought stress, which enriched the gene information database of Brassica campestris L. and laid a foundation for molecular breeding in later stage.更多还原