【摘要】 建立了一种以金、铂纳米粒子修饰的金属有机框架材料（MAP）为标记物的降钙素原（Procalcitonin,PCT）快速定量免疫层析检测方法。本方法以静电吸附法制备的MAP-抗PCT单克隆抗体偶联物为检测探针，将抗PCT多克隆抗体和羊抗鼠IgG分别喷涂硝酸纤维素（NC）膜构建试纸条的检测线和质控线，采用基于信号放大的夹心免疫法定量检测PCT。结果表明，MAP免疫层析试纸条具有灵敏度高、特异性强和稳定性好等优点，利用其检测PCT的动态检测范围为0.61 pg/mL～320 ng/mL，检出限为0.25 pg/mL，日内和日间精密度（RSD)均小于15%。实际样品检测结果表明，本方法仅需微量样本即可检测全血中PCT的浓度，对于炎症反应的早期诊断、监测治疗及预后判断具有重要意义。更多还原

【Abstract】 A rapid quantitative immunochromatographic assay for procalcitonin(PCT) using metal-organic frameworks modified with gold and platinum nanoparticles(MAPs) as labels was established in this work. The detection probe was prepared by conjugating MAPs with anti-PCT monoclonal antibody via an electrostatic adsorption method. Anti-PCT polyclonal antibody and sheep anti-mouse IgG were sprayed onto the nitrocellulose(NC) membrane as the test line and quality control line, respectively, to construct immunochromatographic strip for PCT quantitative detection via signal-amplification-based sandwich immunoassay. The results showed that the MAP-based immunochromatographic test had high sensitivity, high specificity, and good stability. The dynamic range for detection of PCT was 0.61 pg/mL–320 ng/mL, the detection limit was 0.25 pg/mL, and the intra-day and inter-day precision(Relative standard deviation) were less than 15%. The results of real sample analysis showed that a quite low volume of sample was required for detection of PCT in whole blood, which was of great significance for the early diagnosis, monitoring and treatment, and prognosis of inflammation.更多还原