【摘要】 为探究贵州本地2种不同颜色果肉大蕉类胡萝卜素代谢规律，利用高效液相色谱串联质谱法对橙黄肉大蕉与白肉大蕉果实中类胡萝卜素进行定性和定量检测，对类胡萝卜素成分和积累量的种内时序性差异和同时序的种间差异进行分析。结果表明，橙黄肉大蕉与白肉大蕉果肉分别检测到32种和28种类胡萝卜素。橙黄肉大蕉果实发育3个阶段类胡萝卜素积累量均显著高于白肉大蕉，在成熟期橙黄肉大蕉的类胡萝卜素含量是白肉大蕉的7.55倍；橙黄肉大蕉类胡萝卜素的合成主要发生在果实发育中后期，其含量先升高后降低，而白肉大蕉类胡萝卜含量持续降低。橙黄肉大蕉除幼果期叶黄素含量最高外，其他时期均为β-胡萝卜素含量最高，β-胡萝卜、α-胡萝卜素、叶黄素是其主要类胡萝卜素成分；叶黄素、β-胡萝卜素、紫黄质-肉豆蔻酸酯-发酸酯是白肉大蕉的主要类胡萝卜素成分。研究结果为揭示两类大蕉果实类胡萝卜素积累机制奠定基础。更多还原

【Abstract】 In order to explore the metabolism regulation of carotenoid in two different types of local Dajiao in Guizhou Province, HPLC-MS/MS was used to qualitatively and quantitatively detect carotenoids in the fruits of orange-yellow flesh Dajiao BGD and white flesh Dajiao BWD, and the intraspecific temporal and contemporaneous interspecific differences of composition and accumulation of carotenoid were analyzed. The results showed that 32 and 28 kinds of carotenoid were detected in the fruits of BGD and BWD, respectively. The accumulation of carotenoids in the three stages of fruit development of BGD was significantly higher than that of BWD, and the carotenoid content of BGD was 7.54 times of BWD at the maturity stage. The synthesis of carotenoids mainly occurred in the middle and late stages of fruit development in BGD, and its content increased first and then decreased, while the carotenoids content of white-fleshed Dajiao continued to decrease. Except for the lutein content at the young fruit stage, the content of β-carotene was the highest in BGD, and β-carotene, α-carotene and lutein were the main carotenoid components. Lutein, β-carotene, and vioxanthin-myristate-phoroate are the main carotenoid components of BWD. The results of this study lay a foundation for revealing the accumulation mechanism of carotenoids in two Dajiao fruits.更多还原