【摘要】 目的 探讨分泌粒蛋白Ⅱ(secretograninⅡ,SGⅡ)在结直肠神经内分泌肿瘤(colorectal neuroendocrine tumors, CRNETs)中的表达及诊断价值。方法 采用免疫组织化学EnVision两步法检测SGⅡ、突触素(synapsin, Syn)、神经细胞粘附因子(neural cell adhesion molecule, CD56)、嗜铬素A(chromogranin A,CgA)及Ki-67在165例结直肠原发神经内分泌肿瘤(neuroendocrine neoplasms, NENs)及136例非神经内分泌肿瘤(non-neuroendocrine neoplasms, non-NENs)中的表达，比较神经内分泌标记表达的敏感度；并分析CRNETs中SGⅡ、Ki-67、CgA与临床病理特征的相关性。结果 SGⅡ在CRNETs G1、 G2、G3和NEC中的阳性率分别为97.2%(104/107),100%(40/40),100%(5/5),69.2%(9/13),高于non-NENs(1.47%,7/136)中的阳性率(P<0.05)。SGⅡ在CRNETs中的敏感度为95.8%(158/165),与Syn的98.8%(163/165)相当，高于CD56的84.2%(139/165)及CgA的33.9%(56/165),差异有统计学意义(P<0.05)。肿瘤直径、浸润深度、脉管侵犯、神经侵犯及淋巴结转移与SGⅡ的表达呈负相关，与Ki-67表达呈正相关，差异均有统计学意义(P<0.05),而CgA与以上肿瘤性状呈负相关，差异无统计学意义(P>0.05)。结论 SGⅡ在结直肠神经内分泌肿瘤中高表达，是鉴别诊断CRNETs比较可靠的标志物，其表达与肿瘤直径、浸润深度、脉管侵犯、神经侵犯及淋巴结转移呈负相关。更多还原

【Abstract】 Objective To investigate the expression and diagnostic value of secretograninⅡ(SGⅡ) in colorectal neuroendocrine tumors(CRNETs).Methods The expression of SGⅡ、Syn、CD56、CgA and Ki-67 were detected using immunohistochemical EnVision two-step method in 165 neuroendocrine neoplasms(NENs) and 136 non-neuroendocrine neoplasms.Results 1)The positive rates of SGⅡ in CRNETs G1, G2, G3 and NEC were 97.2%(104/107), 100%(40/40), 100%(5/5), 69.2%(9/13), respectively.Higher than the positive rate of non-NENs(1.47%,7/136)(P<0.05);2) The sensitivity of SGⅡ in CRNETs was 95.8%(158/165), which was similar to Syn(98.8%(163/165), higher than that of CD56(84.2%(139/165) and CgA(33.9%).3) Tumor diameter, invasion depth, vascular invasion, nerve invasion and lymph node metastasis were negatively correlated with SⅡ expression and positively correlated with Ki-67 expression, with statistical significance(P<0.05), while CgA was negatively correlated with the above tumor traits, with no statistical significance(P>0.05).Conclusion SGⅡ is highly expressed in colorectal neuroendocrine tumors, which is a reliable marker for the differential diagnosis of CRNETs.Its expression is negatively correlated with tumor diameter, invasion depth, vascular invasion, nerve invasion and lymph node metastasis.更多还原