【摘要】 【目的】为探索适宜草菇生产的菌株保藏10个月的环境条件，保证现有草菇菌株的优良特性。【方法】以‘V9715’草菇为供试菌株，设置保藏温度、保藏基质、添加剂等3个保藏条件因素，从菌丝的萌发与生长、厚垣孢子数量及酶活力等方面分析评价保藏效果，探索不同保藏因素对草菇菌株保藏10个月保藏效果的影响。【结果】在不同保藏条件下保藏10个月，草菇菌丝的萌发和生长情况、厚垣孢子形成早晚、酶活力均存在差异。其中以保藏条件处理C(20℃+玉米芯+液体石蜡)的菌丝萌发率88.30%为最高，萌发天数为2 d，厚垣孢子形成时间是11.5 d，满皿天数4 d为最短，菌丝生长速度1.14 cm/d为最快，其菌丝长势强、气生菌丝浓密度大、菌丝生物量中等；而保藏条件处理A(8℃+棉籽壳+无添加剂)的菌丝生物量最多（0.36 g），但与其他处理差异不显著。处理C(20℃+玉米芯+液体石蜡)的菌丝细胞色素P450氧化还原酶（CPR）、多酚氧化酶（PPO）活力最低，分别为2.16 mg/g FW、36.91 U/g FW，总淀粉酶（AMY）活力最高，为11.88 mg/（min·g） FW。【结论】在处理C(20℃+玉米芯+液体石蜡)保藏条件下，保藏草菇菌株10个月的保藏效果最好。更多还原

【Abstract】 【Objective】To explore the environmental conditions suitable for the preservation of Volvariella volvacea strain for 10 months and ensure the excellent characteristics of the existing V. volvacea strains. 【Method】 Taking V. volvacea‘V9715’as the test strain,three preservation condition factors such as preservation temperature,preservation substrate,and additive were set. The preservation effect was analyzed and evaluated from aspects such as the germination and growth of hyphae,the number of chlamydospores,and enzyme activity to explore the influence of different preservation factors on the preservation effect of V. volvacea strains after 10 months of preservation.【Result】 After 10 months of preservation under different preservation conditions,there were differences in the germination and growth of V. volvacea hyphae,the formation time of chlamydospores,and enzyme activity. Among them,the germination rate of hyphae under preservation condition treatment 3(20℃ + corncob + liquid paraffin) was the highest at 88.30%,the germination days were 2 days,the formation time of chlamydospores is 11.5 days,and growth days to fill petri dish were the shortest at 4 days. The hyphal growth rate was the fastest at 1.14 cm/d. The hyphae have strong growth potential,high density of aerial hyphae,and medium mycelial biomass while the mycelial biomass was the largest(0.36 g) under preservation condition treatment 1(8℃ + cottonseed hull + no additive). There is no significant difference among other treatments. The activities of cytochrome P450oxidoreductase(CPR) and polyphenol oxidase(PPO) of hyphae under treatment 3(20 ℃ + corncob + liquid paraffin) are the lowest,which were 2.16 mg/g FW and 36.91 U/g FW,respectively. The activity of total amylase(AMY) was the highest,which was 11.88 mg/（min·g） FW. 【Conclusion】 Under the preservation condition of treatment 3(20℃ + corncob + liquid paraffin),the preservation effect of V. volvacea strains for 10 months was the best.更多还原