【摘要】 【目的】为丰富血叶兰转录组数据信息，以期帮助兰科植物的功能基因挖掘。【方法】本试验通过Illumina测序平台对两种叶色血叶兰的叶片进行转录组测序和生物信息学分析。【结果】两种叶色血叶兰样品各三个重复总计产出37.23 Gb Clean reads，共获得97 089条Unigene序列，平均长度1 038.76 bp,N50达到1 892 bp。将得到的Unigene和8个公共数据库进行比较，31 095个Unigene获得注释。其中29730条Unigene在NR数据库中获得注释，与铁皮石斛的同源序列最多。与GO数据库进行比对，23 871条Unigene在细胞组分、生物进程和分子功能三大类别中获得注释。通过与KOG数据库的比对，14 791个Unigene获得注释，这些基因的功能主要集中在三大功能类别：普通功能预测、蛋白的翻译后修饰以及信号转导。在两个品系间的差异表达基因共5 791个。与暗紫色叶片相比，绿色叶片中3 120个基因的表达量升高，2 671个基因表达量降低。差异表达基因的KEGG富集结果表明，最显著性富集的是植物与病原菌相互作用，其次是植物MAPK信号途径和植物激素信号转导途径、光合作用-天线蛋白、还有苯丙烷类代谢途径、类黄酮代谢途径、卟啉和叶绿素代谢途径。【结论】研究结果将为深入发掘血叶兰药用植物的功能基因研究奠定一定的基础。更多还原

【Abstract】 【Objective】To enrich transcriptome data of Ludisia discolor in order to assist in its functional gene mining.【Method】Leaves of two kinds of L.discolor cultivars with different leaf colors were sequenced by Illumina Hiseq 2000 and further analyzed by bioinformatics.【Result】37.23 Gb clean reads were generated from three replicates of L. discolor with two leaf colors,which was the de novo assembled into 97089 unigenes with a mean length of 1038.76bp,N50 of 1892 bp. 31095 unigenes were annotated against 8 prominent bioinformatics databases. Within the NR database,a total of 29730 unigenes were primarily annotated from L. discolor;with the most homologous sequences to Dendrobium officinale. 23871 unigenes were annotated in GO database and were divided into three categories:cellular component,biological process and molecular function. There were 14791 unigenes annotated in KOG function categories,which mainly focused on general function prediction,posttranslational modification and signal transduction. A total of 5791 DEGs were screened out from‘XGH’vs‘BX’. Compared with the darkpurple leaves,the expression of 3120 genes increased while the expression of 2671 genes decreased in green leaves.KEGG enrichment analysis showed that the differentially expressed genes were most significantly enriched in plantpathogen interaction;the secondary enriched was MAPK signaling pathway and plant hormone signal transduction,photosynthesis-antenna protein,and the other pathway including phenylpropanoid biosynthesis,flavonoid biosynthesis,porphyrin and chlorophyll metabolism. 【Conclusion】This investigation furnished scientific support for study of functional genes in L. discolor as medicinal plant.更多还原