节点文献

一锅法制备的单链抗体保护荧光金纳米簇及其在细胞成像中的应用（英文）

A Single-chain Antibody for One-pot Fabrication of Luminescent Gold Nanoclusters and Rabies Virus Imaging in Cells

推荐 CAJ下载
PDF下载
不支持迅雷等下载工具，请取消加速工具后下载。

【Author】 ZHANG Chunxia;XI Hualong;SUN Bo;WU Yongge;JIANG Chunlai;YU Xianghui;WU Yuqing;State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University;Institute of Theoretical Chemistry, College of Chemistry, Jilin University;Medical Materials Research and Development Center, Tianjin Baogang Rare Earth Research Institute Co., Ltd.;National Engineering Laboratory for AIDS Vaccine, School of Life Science, Jilin University;

【通讯作者】吴玉清;

【机构】吉林大学化学学院无机与超分子国家重点实验室；吉林大学化学学院理论化学研究所；天津包钢稀土研究院有限责任公司医疗材料研发中心；吉林大学生命科学学院艾滋病疫苗国家工程实验室；

【摘要】由于易碎抗体的存在，使得单锅法制备发光金属纳米团簇面临很大挑战。本研究提出了一种稳定的单链抗体（scFv57R-ATS），用于制备发光金纳米团簇（AuNCs@scFv57R-ATS）和在活细胞中快速、敏感地检测狂犬病病毒。本文设计的AuNCs@scFv57R-ATS在被修饰的HeLa细胞中能特异性识别抗原RV，这推动了金属纳米簇荧光探针对于抗原的靶向示踪及治疗示范。更多还原

【Abstract】 The fragile antibody leads to a great challenge as a scaffold to fabricate the luminescent metal nanoclusters using one-pot method. This study presents a stable single-chain anti-body(scFv57R-ATS) for the fabrication of luminescent gold nanoclusters(AuNCs@scFv57R-ATS) and a quick, sensitive rabies virus detection in living cells.In this paper, AuNCs@scFv57R-ATS was designed to specifically recognize antigen RV in modified HeLa cells, which promoted the demonstration of metal nanocluster fluorescent probes for antigen targeting and therapy.更多还原

【关键词】金纳米簇； scFv57R-ATS；狂犬病毒；
【Key words】 gold nanoclusters； scFv57R-ATS； rabies virus；

【基金】 Supported by National Natural Science Foundation of China(21875085,21373101);Jilin Provincial Science and Technology Development Plan Project(20230204040YY);The Innovation&Open Program of the State Key Laboratory of Supramolecular Structure and Materials,Jilin University(sklssm2023017)~~

【文献出处】发光学报 ,Chinese Journal of Luminescence , 编辑部邮箱 ,2024年10期

【分类号】TB383.1;O657.3;O482.31
【下载频次】19

知网节下载

节点文献中：

本文链接的文献网络图示:

本文的引文网络

节点文献