【摘要】 目的 研究慢性鼻窦炎伴鼻息肉（CRSwNP）患者鼻息肉中白细胞介素10(IL-10)、IL-33的表达水平，及外源性IL-10刺激对嗜酸性粒细胞型CRSwNP(eosinophilicCRSwNP,ECRSwNP)中IL-33表达水平的影响。方法 手术获取ECRSwNP 12例、非嗜酸性粒细胞型CRSwNP(non-eosinophilic CRSwNP,nECRSwNP)8例及12例正常鼻黏膜组织，采用酶联免疫吸附法（ELISA）测定组织中IL-10及IL-33表达水平。另收集10例ECRSwNP样本，以含或不含100 ng/ml IL-10的培养基分别培养同一患者样本24 h后，ELISA及免疫组化检测IL-33表达量。结果 IL-10在ECRSwNP组、nECRSwNP组、对照组中表达无显著差异（P>0.05）。IL-33在ECRSwNP组中的表达水平高于对照组，差异有统计学意义（P=0.0043）。在ECRSwNP组中，IL-10与IL-33表达水平呈显著负相关（r=-0.7470,P=0.0052）；在nECRSwNP组及对照组中两者无明显相关性（P>0.05）。相比于空白对照组，IL-10刺激组中IL-33的表达显著下降（P=0.0197）、免疫组化观察到IL-33阳性细胞显著减少（P=0.0183）。结论 在ECRSwNP中IL-33的表达量显著增高，IL-10与IL-33负相关。外源性IL-10可以抑制ECRSwNP中IL-33的表达。更多还原

【Abstract】 OBJECTIVE To investigate the expression levels of interleukin-10(IL-10) and IL-33 in patients with chronic rhinosinusitis with nasal polyps(CRSwNP) and the effect of exogenous IL-10 stimulation on the expression level of IL-33 in eosinophilic CRSwNP(ECRSwNP). METHODS The expression levels of IL-10 and IL-33 in nasal polyp stissues of 12 patients in ECRSwNP group, 8 patients in non-eosinophilic CRSwNP(nECRSwNP) group, and normal nasal mucosa of 12healthy controls were determined by ELISA. Another 10 ECRSwNP samples were collected and cultured in medium with or without 100 ng/ml IL-10 for 24 hours, and then the expression of IL-33 was detected by ELISA and immunohistochemistry.RESULTS There was no significant difference in expression of IL-10 among ECRSwNP group, nECRSwNP group and healthy control group(P >0.05). The expression level of IL-33 in ECRSwNP group was higher than that in healthy control group, and the difference is statistically significant(P =0.0043). The expression level of IL-10 and IL-33 was a significant negative correlation in ECRSwNP group(r =-0.7470, P =0.0052). There was no significant correlation in nECRSwNP group and control group(P >0.05). Compared with the control group, the expression level of IL-33 in the IL-10-stimulated ECRSwNP group was significantly decreased(P =0.0197), and a significant decrease of IL-33 positive cells was observed in immunohistochemistry(P =0.0183). CONCLUSION IL-33 expression is significantly increased in ECRSwNP, and IL-10 is negatively correlated with IL-33. Exogenous IL-10 can inhibit IL-33 expression in ECRSwNP.更多还原