【摘要】 为研究减数分裂相关基因Spo11(meiotic protein covalently bound to DSB homolog)在罗氏沼虾(Macrobrachium rosenbergii)卵巢发育中的调控作用，采用RACE技术、荧光定量PCR、原位杂交和RNA干扰等方法，对罗氏沼虾Spo11基因(MrSpo11)及其编码氨基酸的分子特征、MrSpo11基因的组织表达、分布和生物学功能进行了研究。结果表明：MrSpo11基因cDNA序列全长为2 298 bp,其中，5′端和3′端非编码区分别为457、701 bp,开放阅读框为1 140 bp,共编码379个氨基酸残基；MrSpo11基因在罗氏沼虾鳃中的相对表达量最高，在卵巢、肝胰腺和心脏中表达量较高，在脑、眼和肌肉中微量表达；MrSpo11基因在罗氏沼虾卵巢发育Ⅰ期相对表达量最高，在卵巢发育Ⅲ期次之，在Ⅱ期和Ⅳ期表达量最低；MrSpo11基因在卵黄发生前期、中期、晚期卵母细胞的细胞质，以及卵黄发生早期卵母细胞的细胞质和细胞核中均有表达；注射dsRNA后第2、4天，试验组MrSpo11基因的表达量分别比对照组下降45.8%、11.6%,注射dsRNA后第4天，试验组卵巢发育成熟度略低于对照组。研究表明，MrSpo11基因参与了罗氏沼虾卵巢发育过程，本研究结果可为进一步探究罗氏沼虾卵巢发育分子调控机制提供有益参考。更多还原

【Abstract】 In order to investigate the regulatory role of meiotic protein covalently bound to DSB homolog(MrSpo11) in ovarian development of Malaysian prawn(Macrobrachium rosenbergii), the molecular characteristics, encoded amino acid, tissue expression, distribution and biological function of MrSpo11 gene were investigated in Malaysian prawn by RACE technique, fluorescence quantitative PCR, in situ hybridization and RNA interference. The results showed that the MrSpo11 cDNA sequence had total length of 2 298 bp, with the 5′end non-coding region of 457 bp, the 3′ end non-coding region of 701 bp, and the open reading frame of 1 140 bp encoding 379 amino acid residues. There was the maximal relative expression level of MrSpo11 gene in gill, higher level in ovary, hepatopancreas and heart, and slightly expression level in brain, eye and muscle. The maximal relative expression level of MrSpo11 gene was observed in stage Ⅰ of ovarian development, followed by stage Ⅲ, and the minimal level in stage Ⅱ and stage Ⅳ. MrSpo11 gene was showed to be expressed in the cytoplasm of pre-vitellogenesis, mid-vitellogenesis and late vitellogenesis oocytes and in the cytoplasm and nucleus of early vitellogenesis oocytes. The expression of MrSpo11 gene was decreased in experimental group by 45.8% and 11.6% on 2nd and 4th day after dsRNA injection, respectively, compared with control group, with slightly lower ovarian development maturity in the experimental group than that in the control group in 4 days of injection, indicating that MrSpo11 gene is involved in the ovarian development. The finding lays a foundation for further research on the molecular regulation mechanism of ovarian development of Malaysian prawn.更多还原