【摘要】 目的：观察健脾润肤汤对人角质形成细胞炎症状态下丝聚蛋白（FLG）、半胱氨酸天冬氨酸蛋白酶-14(Caspase-14)及相关细胞因子表达的影响。方法：应用Western Blot和ELISA检测白细胞介素（IL）-4刺激前、后人角质形成细胞FLG、Caspase-14、IL-1b、IL-13、IL-17、肿瘤坏死因子-α(TNF-α)的表达差异。结果：经健脾润肤汤含药血清高浓度组孵育后FLG及Caspase-14蛋白表达均较空白组显著升高（P<0.05）。与模型组比较，高浓度组IL-1b、TNF-α表达显著升高（P<0.05）,IL-13、IL-17表达显著降低（P<0.05）；低浓度组IL-13、IL-17表达显著降低（P<0.05）,TNF-α表达显著升高（P<0.05）。高浓度组IL-17表达较低浓度组显著升高（P<0.05）。结论：健脾润肤汤含药血清可以提高炎症环境下人角质形成细胞FLG和Caspase-14的表达，增强皮肤屏障功能；进而减少特应性皮炎发病中细胞因子的进一步产生，提高IL-1b、TNF-α表达，降低IL-13、IL-17水平，修复Th1/Th2平衡，减轻炎症程度。更多还原

【Abstract】 Objective: To observe the effects of Jianpi Runfu Decoction on the expressions of filaggrin(FLG), Caspase-14 and related cytokines in keratinocytes with inflammation. Methods: The expression differences of FLG, Caspase-14, interleukin(IL)-1b, IL-13, IL-17 and TNF-α in keratinocytes before and after stimulation of IL-4, were detected by Western Blot and ELISA.Results: The expressions of FLG and Caspase-14 in keratinocytes were higher than those in the blank group after incubation with the low concentration medicated serum of Jianpi Runfu Decoction(P<0.05). Compared with the model group, the expression of IL-1b and TNF-α in the high concentration group was significantly increased(P<0.05), while the expression of IL-13 and IL-17was significantly decreased(P<0.05), the expression of IL-13 and IL-17 in the low concentration group was significantly decreased(P<0.05), while the expression of TNF-α was significantly increased(P<0.05). The expression of IL-17 in the high concentration group was significantly higher than that in the low concentration group(P<0.05). Conclusion: The serum containing Jianpi Runfu Decoction can increase the expression of FLG and Caspase-14 in keratinocytes with inflammation, enhance the skin barrier function and reduce the cytokines relate to AD. It could increase the expression of IL-1b and TNF-α, while reduce IL-13 and IL-17 levels,the balance between Th1/Th2 would be repaired, and the degree of inflammation can be reduced.更多还原