【摘要】 目的:检测原发性高血压大鼠肠上皮细胞来源的外泌体中具有差异表达的miRNA,并对其靶基因进行富集分析。方法:选用自发性高血压大鼠(SHR)、魏-凯二氏大鼠(WKY)各3只作为研究对象，通过高通量测序方法检测大鼠肠上皮细胞来源外泌体中miRNA的表达，筛选两组大鼠差异表达miRNA,预测其靶基因，应用生物学功能软件对靶基因进行基因本体论(GO)和京都基因与基因组百科全书(KEGG)分析。结果:与WKY组相比，SHR组共有37个差异表达的miRNA(均P<0.05),32个上调，5个下调，其中值得关注的有：rno-miR-208b-3p、rno-miR-134-5p、rno-miR-93-3p、rno-miR-378b、rno-miR-96-5p、rno-miR-210-3p、rno-miR-208a-3p。共预测miRNA下游靶基因10 662个，预测到的靶位点数共34 127个，GO富集主要集中在膜结合细胞器、蛋白质结合、正向调节的生物过程等。KEGG富集主要集中在MAPK、mTOR、Ras及TNF信号通路等。结论:原发性高血压大鼠肠上皮细胞来源外泌体miRNA显著差异表达，其靶基因可能通过细胞生长分化、血管生成、代谢功能，参与MAPK、mTOR、Ras及TNF信号通路，从而影响高血压疾病的发生发展。更多还原

【Abstract】 Objective:To detect differentially expressed miRNAs in exosomes derived from intestinal epithelial cells of essential hypertensive rats, and to enrich and analyze their target genes. Methods: Three Spontaneously hypertensive rats(SHR) and three Wistar-Kyoto rats(WKY) were selected as the research objects. The expression of miRNA in exosomes derived from intestinal epithelial cells of rats was detected by high-throughput sequencing. The differentially expressed miRNAs in the two groups of rats were screened and their target genes were predicted. The target genes were analyzed by Gene ontology(GO) and Kyoto encyclopedia of genes and genomes(KEGG) using biological function software. Results: Compared with the WKY group, there were 37 differentially expressed miRNAs in the SHR group(All P<0.05), 32 miRNAs were up-regulated and 5 miRNAs were down-regulated. Among them, rno-miR-208b-3p, rno-miR-134-5p, rno-miR-93-3p, rno-miR-378b, rno-miR-96-5p, rno-miR-210-3p, and rno-miR-208a-3p were noteworthy. A total of 10 662 miRNA downstream target genes were predicted, and a total of 34 127 target sites were predicted. GO enrichment was mainly concentrated in membrane-bound organelles, protein binding, and positively regulated biological processes. KEGG enrichment was mainly concentrated in MAPK, mTOR, Ras and TNF signaling pathways. Conclusion: The expression of miRNA in exosomes derived from intestinal epithelial cells of essential hypertensive rats was significantly different. Its target genes may be involved in MAPK, mTOR, Ras and TNF signaling pathways through cell growth and differentiation, angiogenesis and metabolic function, thus affecting the occurrence and development of hypertensive diseases.更多还原