【摘要】 黏面子是以玉米面为原料发酵制成的东北地区特有的发酵食品。为探讨从黏面子中分离出的植物乳植杆菌H8是否对小鼠氧化损伤具有改善作用，建立了D-半乳糖致氧化损伤的衰老小鼠模型，并对其可能的信号机制进行研究。取32只昆明小鼠，每天注射200 mg/kg的D-半乳糖致小鼠氧化损伤，同时灌胃浓度为1.0×10¹⁰ CFU/mL的植物乳植杆菌H8,持续8周，观察H8对氧化损伤小鼠血清和肝脏的作用情况。结果表明：H8可通过提高器官指数保护小鼠组织免受氧化应激损伤，并显著降低了血清和肝脏中氧化应激水平，包括一氧化氮和丙二醛，同时提高了血清和肝脏中抗氧化指标，包括超氧化物歧化酶、谷胱甘肽过氧化物酶和还原型谷胱甘肽的活性；此外，H&E切片染色发现，植物乳植杆菌H8可以减少D-半乳糖诱导的小鼠肝、脾、脑和结肠组织的损伤；同时，植物乳植杆菌H8可以激活sirtuin1 （SIRT1）和sirtuin3 （SIRT3）,并激发Nrf2-ARE途径诱导抗氧化酶的mRNA和蛋白的表达水平；并且，SIRT1可以促进p53的泛素化和降解，并减少活性氧的生成。研究结果表明，植物乳植杆菌H8的抗氧化作用可能与SIRT1/SIRT3/Nrf2信号通路的上调有关，研究了SIRT3通路和乳植杆菌属之间的潜在关联。本研究旨在为开发抗氧化性益生菌产品提供理论参考。更多还原

【Abstract】 Nianmianzi is a unique fermented food in Northeast China which is fermented with corn flour as raw material. In order to explore whether Lactiplantibacillus plantarum H8 isolated from Nianmianzi can improve the oxidative damage in mice, an aging mouse model of oxidative damage induced by D-galactose was established, and its possible signal mechanism was studied. 32 Kunming mice were injected with 200 mg/kg D-galactose every day to induce oxidative damage in mice, and at the same time, Lactiplantibacillus plantarum H8 with a concentration of 1.0×10¹⁰ CFU/mL was gavaged for 8 weeks to observe the effect of H8 on serum and liver of mice with oxidative damage. According to the findings, H8 could prevent oxidative stress in mice tissues by raising the organ index. It could also dramatically lower oxidative stress levels in serum and liver, including NO and MDA, and increase the activity of antioxidant indices in serum and liver, like SOD, GSH-Px, and GSH. Furthermore, Lactiplantibacillus plantarum H8 was shown to be able to lessen the harm that D-galactose caused to mice’s liver, spleen, brain, and colon tissue using H&E section staining. Meanwhile, Lactiplantibacillus plantarum H8 had the ability to promote the expression of antioxidant enzymes generated by the Nrf2-ARE pathway at the mRNA and protein levels, as well as activate sirtuins 1（SIRT1） and sirtuins 3. SIRT1 could encourage the ubiquitination and destruction of p53 and lessen the production of reactive oxygen species. The findings suggested that the up-regulation of the SIRT1/SIRT3/Nrf2 signaling pathway might be connected to the antioxidant activity of Lactiplantibacillus plantarum H8, and a possible correlation between the SIRT3 signaling pathway and Lactiplantibacillus was investigated. The goal of this study wa to provide theoretical reference for developing antioxidant probiotic products.更多还原