【摘要】 目的 观察融化后新鲜冰冻血浆、新鲜液体血浆在2～6℃储存过程中部分凝血因子活性和蛋白含量的变化，为探索FFP解冻后2～6℃储存的合适时间提供参考。方法 小包装/大包装冻融组新鲜冰冻血浆分别于融化后d1、2、3、4、5、6、7检测凝血因子FⅤ活性(FⅤ∶C)、凝血因子FⅧ活性(FⅧ∶C)、纤维蛋白原(Fib)、总蛋白(TP)、白蛋白(Alb)的含量；新鲜液体血浆分别于制备后d1、2、3、4、5、6、7及制备后d1、6、11、16、21、26、31检测FⅤ∶C、FⅧ∶C、Fib、TP、Alb的含量。结果 FFP组FⅧ∶C随着融化后储存时间的延长逐渐下降(P<0.05),两组在d7分别下降了37.4%和47.6%;FⅤ∶C、Fib、TP、Alb差异无统计学意义(P>0.05)。新鲜液体血浆组FⅧ∶C随着融化后储存时间的延长逐渐下降(P<0.05);FⅤ∶C储存7 d组差异无统计学意义(P>0.05),储存31d组逐渐下降(P<0.05); Fib、TP、Alb差异无统计学意义(P>0.05)。结论 2～6℃储存7 d的融化FFP虽然FⅧ∶C有所下降，但稳定在52.4%,符合临床需求。更多还原

【Abstract】 Objective To observe the changes of coagulation factor activity and protein content of the thawed fresh frozen plasma(FFP) and fresh liquid plasma(FLP) during storage at 2-6℃, and to provide reference for exploring the appropriate storage time of FFP at 2-6℃ after thawing. Methods The small-thaw group and the large-thaw group were respectively detected for the activity of coagulation factor FⅤ(FⅤ∶C) and FⅧ(FⅧ∶C), and the levels of fibrinogen(Fib), total protein(TP) and albumin(Alb) in TTP at 1, 2, 3, 4, 5, 6 and 7 days after thawing. And the FLP was detected for FⅤ∶C, FⅧ∶C, Fib, TP and Alb at 1, 2, 3, 4, 5, 6, 7 days and 1, 6, 11, 16, 21, 26 and 31 days after preparation, respectively. Results In FFP group, FⅧ∶C decreased gradually with the prolongation of storage time after melting(P<0.05), and decreased by 37.4% and 47.6% respectively in the two groups on the 7th day. There was no statistical difference in FⅤ∶C, Fib, TP and Alb(P>0.05). In FLP group, FⅧ∶C decreased gradually with the prolongation of storage time after melting(P<0.05). There was no statistical difference in FⅤ∶C in 7-day storage group(P>0.05), but it decreased gradually in 31-day storage group(P<0.05). There was no statistical difference in Fib, TP and Alb(P>0.05). Conclusion Although FⅧ∶C is decreased in thawed FFP stored at 2-6℃ for 7 days, it is still about 52.4%, which should be able to play a normal role in clinical practice.更多还原