【摘要】 为了解鼓槌石斛、细茎石斛和球花石斛种子无菌播种快繁技术，以3种石斛种子为研究对象，探讨不同培养基、激素种类以及浓度配比对3种石斛种子无菌萌发、原球茎增殖和种苗壮苗生根的影响。结果表明：3种石斛种子的萌发率最高均在1/2MS基础培养基和添加10%马铃薯的1/2MS培养基上；鼓槌石斛和球花石斛原球茎增殖培养基以1/2MS+10%马铃薯+0.5 mg/L 6-BA+0.2 mg/L NAA最佳，细茎石斛原球茎增殖培养基以1/2MS+10%马铃薯+1.0 mg/L 6-BA+0.2 mg/L NAA最佳；鼓槌石斛生根壮苗培养基以1/2MS+0.10 mg/L IBA+10%马铃薯+2.0%蔗糖+0.6%琼脂最佳，球花石斛和细茎石斛的生根壮苗培养基以1/2MS+0.50 mg/L IBA+10%马铃薯+2.0%蔗糖+0.6%琼脂最佳。更多还原

【Abstract】 To understand the Dendrbium chrysotoxum,Dendrobium moniliforme and Dendrobium thyrsiflorum seed sterile plant rapid propagation techniques, in this paper, three kinds of Dendrobium seeds as the research object, discusses different types of medium, hormone and concentration ratio of 3 kinds of Dendrobium sterile seed germination, protocorm proliferation and seedling seedling rooting effect.The results showed that the highest germination rate of the 3 Dendrobium seeds was on 1/2MS base culture and 1/2MS medium with 10% potato.Dendrobium chrysotoxum and Dendrobium thyrsiflorum were optimized with 1/2MS +10% potato +0.50 mg/L 6-BA+0.2 mg/L NAA,while Dendrobium moniliforme was optimized with 1/2MS +10% potato +1.0 mg/L 6-BA+0.2 mg/L NAA.The best rooting and seedling medium of Dendrobium chrysotoxum moniliforme was 1/2MS+0.10 mg/L IBA+10% potato +2.0% sucrose +0.6% AGAR,and the best rooting and seedling medium of Dendrobium thyrsiflorum and Dendrobium moniliforme was 1/2MS+0.50 mg/L IBA+10% potato +2.0% sucrose +0.6% AGAR.更多还原