【摘要】 为研究新型鹅星状病毒(Novel goose astroviruses, N-GAstV)的遗传进化特征，获得了鹅星状病毒安徽分离株AstV/AHHX的全长基因，为7 251 nt。测序分析显示：AstV/AHHX具有典型的新型鹅星状病毒基因组特征。AstV/AHHX与分离株GDZJ37以及山东分离株G538和G576的遗传距离较近；同时与毒株GDZJ37、G538和G576的ORF2氨基酸序列同源性达100%,ORF1a和ORF1b的同源性也有99.6%以上，推测它们由同一毒株演化而来。ORF2氨基酸序列位点分析显示有12高变异位点，分别是第36(Y/S/H)、60(V/I)、224(T/A)、228(A/S/T)、229(P/Q)、289(T/A)、376(T/A)、456(D/E)、540(Q/L)、608(S/T)、610(E/D/G)、614(N/A/T)位氨基酸。其中第224、228、229、608、614位的氨基酸突变可能导致病毒蛋白质的构象及功能发生变化，进而改变病毒的致病能力。更多还原

【Abstract】 In order to study the genetic and evolutionary characteristics of a novel goose astrovirus(N-GAstV),the full-length genes of Anhui strain AstV/AHHX were cloned in this study, and it was 7 251 nt.Sequencing analysis showed that AstV/AHHX had typical characteristics of N-GAstV.The phylogenetic tree showed that AstV/AHHX was relatively close to GDZJ37 isolates from China, G538 and G576 isolates from Shandong Province in recent years.The homology of ORF2 with GDZJ37,G538 and G576 was 100%,and the homology of ORF1a and ORF1b was more than 99.6%.Presumably they evolved from the same strain.Amino acid sequence analysis of ORF2 revealed 12 highly variable sites.They were the amino acid sites, 36(Y/S/H),60(V/I),224(T/A),228(A/S/T),229(P/Q),289(T/A),376(T/A),456(D/E),540(Q/L),608(S/T),610(E/D/G),614(N/A/T).Amino acid mutations at position 224,228,229,608 and 614 may lead to changes in the conformation and function of viral proteins, then the pathogenic ability of the virus was altered.更多还原