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中性蛋白酶高温酶解条件下猪粪资源化利用中养分的变化规律
Changes Rule of Nutrients During the Resource Utilization of Pig Manure Under High Temperature Enzymatic Hydrolysis with Neutral Protease
【摘要】 以酶工程及酶动力学为技术依托及科学依据,将猪粪加玉米秸秆组合作为底物,添加2%中性蛋白酶,在生化反应釜中80℃酶解3 h,底物含水量降低至56.01%,同比下降2.34%;大分子有机质含量降低至63.79%,酶解后,A、B处理的底物有机质含量分别降低了14.19%、15.06%;植物可利用大量元素提高至6.87%,符合NY 525—2012有机肥标准;速效磷、速效钾、全盐、总腐殖酸呈下降趋势;蛔虫卵死亡率达到92%,种子发芽指数达到54%,达到有机肥腐熟标准。
【Abstract】 Based on enzyme engineering and enzyme kinetics as the technical support and scientific basis, the combination of pig manure and corn stover was used as the substrate, 2% neutral protease was added, and the water content of the substrate was reduced to 56.01% at 80°C for 3 h in a biochemical reactor, a year-on-year decrease of 2.34%.The content of macromolecular organic matter was reduced to 63.79%,and after enzymatic hydrolysis, the organic content of substrates treated A and B reached 14.19% and 15.06%,respectively.The use of a large number of elements in plants had been increased to 6.87%,which met the NY 525-2012 organic fertilizer standard.Available-available phosphorus, available potassium, total salt and total humic acid showed a downward trend.The mortality rate of roundworm eggs reached 92%,and the seed germination index reached 54%,which reached the standard of organic fertilizer rot.
【Key words】 Pig manure; Neutral protease; High temperature enzymatic hydrolysis; Organic fertilizer; Nutrient; Change rule;
- 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2024年04期
- 【分类号】S141
- 【下载频次】18