【摘要】 [目的]明确高效提取金钱槭基因组DNA的方法。[方法]以金钱槭幼嫩叶片为材料，采用4种不同方法提取金钱槭基因组DNA,并通过紫外分光光度计法、琼脂糖凝胶电泳检测、限制性内切酶酶切和扩增保守基因片段等方法比较不同DNA的提取效率。[结果]高盐低pH法因操作时间较长导致DNA损失较多，提取到的DNA虽然杂质含量较低，但浓度也较低。SDS法提取效果不佳，提取到的DNA含量少且完整性差。尿素法提取的DNA质量最差，降解严重，难以用于后续分子生物学研究。改良CTAB法可以大量提取能够用于酶切及PCR扩增的DNA。[结论]金钱槭DNA高效提取方法的确定可以为相关的分子生物学研究提供科学依据和技术参考。更多还原

【Abstract】 [Objective]To determine efficient method for extracting genomic DNA from D. sinensis.[Method]Four methods were used to extract genomic DNA from young leaves of D.sinensis.The efficiency of genomic DNA extraction was assessed by ultraviolet spectrophotometer, agarose gel electrophoresis, restriction enzyme digestion, and amplification of conserved gene fragments.[Result]The results showed that the high-salt, low-pH method led to more DNA loss due to the extended operation time, resulting in low concentration of DNA with low impurity content. The SDS method had a poor extraction efficiency, yielding DNA with low content and poor integrity. The urea method produced the worst DNA extraction, with severe degradation that renders it unsuitable for molecular biology research. The improved CTAB method successfully extracted a large amount of DNA suitable for enzyme digestion and PCR amplification. [Conclusion]The determination of an efficient DNA extraction method will provide scientific basis and technical references for related molecular biology research of D. sinensis.更多还原