【摘要】 高内涵筛选（HCS）技术整合了自动化高速成像与单细胞定量分析。它可以大大加速细胞荧光成像中的数据采集，是中医药研究中一项强大的研究技术。越来越多的实验室和平台，包括中医实验室，已经开始使用HCS。然而，该技术在中医药研究中仍处于起步阶段，在相关概念、仪器配置和分析方法方面缺乏足够的经验。为提高中医药领域研究人员对HCS的认识，本文结合本团队以往的研究经验，总结了HCS的概念、软硬件配置、整体研究过程，以及HCS在中医药研究中的常见问题及相关解决方案，提供了若干研究实例和对未来的展望，旨在为中医药研究中的HCS提供技术指导。更多还原

【Abstract】 High-content screening(HCS) technology combines automated high-speed imaging hardware and single-cell quantitative analysis. It can greatly accelerate data acquisition in cellular fluorescence imaging and is a powerful research technique in traditional Chinese medicine(TCM). An increasing number of laboratories and platforms, including TCM laboratories, have begun utilizing HCS systems. However, this technology is still in its infancy in TCM research and there is a lack of sufficient experience with the associated concepts, instrument configurations, and analysis methods. To improve the understanding of HCS among researchers in the field of TCM, this paper summarizes the concept of HCS, software and hardware configuration, the overall research process, as well as common problems and related solutions of HCS in TCM research based on our team’s previous research experience, providing several research examples and an outlook on future perspectives, aiming to provide a technical guide for HCS in TCM research.更多还原