【摘要】 本文以危害油樟的东方绢金龟为研究对象，采用Illumina Miseq高通量测序技术与纯培养技术相结合的方法，分析东方绢金龟肠道细菌的多样性和群落结构，并利用不同的功能选择培养基筛选功能菌株，丰富特有的功能菌种资源。Illumina Miseq测序结果表明，东方绢金龟肠道细菌隶属于15门、25纲、57目、98科、141属、188种，优势门为厚壁菌门，优势属为毛螺菌科的未分类属与丹毒杆菌属；可培养肠道细菌结果分析表明，128株菌隶属于4门、5纲、6目、7科、10属，优势门为朊细菌门，优势属为沙雷氏菌属；运用功能选择培养基筛选出19株产蛋白酶功能菌、13株产酯酶功能菌、9株产脂肪酶功能菌。研究结果解析了东方绢金龟肠道细菌的多样性，为今后深入了解东方绢金龟肠道细菌在调控东方绢金龟与寄主植物油樟互作关系中的作用奠定基础。更多还原

【Abstract】 In this paper, adults of Maladera orientalis damaging Camphora longepaniculata were used as the material and a approach combined with Illumina Miseq high-throughput sequencing and pure culture techniques was adopted to analyse the intestinal bacterial diversity of Maladera orientalis and community structure. Different function selecting mediums were used to screen out functional bacterial strains and enrich specific functional strain resources. Illumina Miseq sequencing showed that the intestinal bacteria of Maladera orientalis include 188 species belonging to 141 genera from 98 families in 57 orders under 25 classes of 15 phyla, with Firmicutes as the dominate phylum, unclassified Lachnospiraceae and Erysipelatoclostridium as the dominate genera. Analysis showed that the culturable intestinal bacteria include 128 strains belonging to 10 genera from 7 families in 6 orders under 5 classes of 4 phyla, with Prion as the dominant phylum and Serratia as the dominant genus. 19 protease producing strains, 13 esterase producing strains and 9 lipase producing strains were screened out with function selecting mediums. This study analyzed the intestinal bacterial diversity of Maladera orientalis and could lay a foundation for further understanding the role of the intestinal bacteria in regulating the interaction between Maladera orientalis and the host plant Camphora longepaniculata.更多还原