【摘要】 目的 探讨六灵解毒丸对金黄色葡萄球菌致大鼠急性咽炎的作用及机制。方法 采用大鼠咽喉部注入1×10⁹CFU·mL^-1的金黄色葡萄球菌溶液的方法复制急性咽炎大鼠模型。将SD大鼠随机分为空白组、模型组、蓝芩口服液组(5 mL·kg^-1)及六灵解毒丸低、中、高剂量组(4.375、8.750、17.500 mg·kg^-1)，每组10只。各组大鼠灌胃给药，每日1次，连续7 d。造模及给药期间每天观察并记录大鼠一般情况，并对咽喉局部炎症情况进行评分；采用苏木素-伊红（HE）染色法观察大鼠咽部组织病理学变化；ELISA法检测大鼠血清白细胞介素1β（IL-1β）、白细胞介素6（IL-6）、肿瘤坏死因子α（TNF-α）含量；免疫组化法及Western Blot法检测大鼠咽部组织中IL-1β、IL-6、TNF-α蛋白表达水平。结果 与空白组比较，模型组大鼠咽喉红肿程度显著增加，炎症评分显著升高（P<0.01），造模后第5～7天的体质量显著降低（P<0.05,P<0.01），咽部组织病理学评分显著升高（P<0.01），血清炎症因子IL-1β、IL-6、TNF-α水平均显著上升（P<0.01），咽部组织中IL-1β、IL-6、TNF-α蛋白表达水平显著升高（P<0.01）。与模型组比较，蓝芩口服液组及六灵解毒丸低、中、高剂量组大鼠的咽喉红肿明显减轻，炎症评分显著降低（P<0.01），血清IL-1β、IL-6、TNF-α水平均显著下降（P<0.01）；蓝芩口服液组及六灵解毒丸中、高剂量组大鼠造模后第7天的体质量显著增加（P<0.01），咽部组织病理学评分及IL-1β、IL-6、TNF-α蛋白表达水平均显著降低（P<0.05,P<0.01）。结论 六灵解毒丸可显著改善急性咽炎大鼠的症状及咽部组织炎症病理改变，其机制可能与下调IL-1β、IL-6、TNF-α等炎性因子表达水平有关。更多还原

【Abstract】 Objective To investigate the effect and mechanism of Liuling Jiedu Pills on acute pharyngitis caused by Staphylococcus aureus in rats. Methods The rat model of acute pharyngitis was replicated using the method of injecting 1×10⁹CFU·mL^-1of Staphylococcus aureus solution into the pharynx of rats. SD rats were randomly divided into a blank group,a model group,a Lanqin Oral Solution group(5 mL·kg^-1),and a low-,medium-,and highdose group of Liuling Jiedu Pills(4.375,8.750,and 17.500 mg·kg^-1),with 10 rats in each group. Rats in each group were administered the drug by gavage once a day for 7 days. The general conditions of the rats were observed and recorded every day during the modeling and drug administration periods, and the local inflammation in the pharynx was scored;histopathological changes in the pharynx of the rats were observed by hematoxylin-eosin（HE）staining; serum interleukin 1β（IL-1β）, interleukin 6（IL-6）, tumor necrosis factor α（TNF-α）, and tumor necrosis factor-α（TNF-α）were detected by ELISA. Immunohistochemistry and Western Blot were used to detect the protein expression levels of IL-1β,IL-6 and TNF-α in rat pharyngeal tissue. Results Compared with the blank group,rats in the model group had significantly increased pharyngeal erythema,significantly higher inflammation scores（P<0.01）, significantly lower body mass on days 5-7 after modeling（P<0.05, P<0.01）, significantly higher pathological scores（P<0.01）,significantly higher levels of the serum inflammatory factors IL-1β,IL-6,and TNF-α（P<0.01）,and significantly higher pharyngeal tissues showed significantly higher levels of IL-1β,IL-6, and TNF-α proteins（P< 0.01）. Compared with the model group, the pharyngeal erythema was significantly reduced in the Lanqin Oral Solution group and the low-,medium-and high-dose groups of Liuling Jiedu Pills,and the inflammation scores were significantly reduced（P<0.01）,and the serum levels of IL-1β,IL-6,and TNF-α were significantly reduced（P<0.01）;the body mass of the rats in the Lanqin Oral Solution group,and in the medium-and high-dose groups of Liuling Jiedu Pills, were significantly increased on the seventh day of the modeling（P<0.01）;the histopathological scores and the levels of IL-1β,IL-6 and TNF-α proteins in pharyngeal tissue were significantly decreased（P<0.05, P<0.01）. Conclusion Liuling Jiedu Pills can significantly improve the symptoms and inflammatory pathological changes of pharyngeal tissues in rats with acute pharyngitis,and its mechanism may be related to the down-regulation of the expression levels of inflammatory factors such as IL-1β,IL-6,and TNF-α.更多还原