【摘要】 目的：采用Box-Behnken效应面法优化灵芝-巴豆双向发酵制备腺苷的最优参数，同时探究巴豆发酵品“灵巴菌质”的抗氧化活性。方法：通过单因素实验和Plackett-Burman实验选择对腺苷产量有显著影响的因素。此外，通过Box-Behnken实验设计，以腺苷含量为指标，研究并优化了灵芝发酵过程中初始含水量、接种量、发酵时间和初始发酵pH值之间的相互作用；通过还原力能力、DPPH自由基清除能力、羟自由基清除能力对“灵巴菌质”进行抗氧化评价。结果：“灵巴菌质”制备的最佳工艺条件为：初始含水量为80%，接种量为0.25 mg/g，发酵时间为27 d，发酵pH5，此时的腺苷的含量为2.07 mg/g。抗氧化结果表明，“灵巴菌质”水提物和醇提物均具有不同程度的还原力和对DPPH自由基、羟自由基的清除作用，水提物的抗氧化略强一些；二者的抗氧化能力均大于巴豆水提物和醇提物。结论：本研究建立的模型可对“灵巴菌质”中腺苷含量进行分析和预测，为巴豆资源的开发和利用提供依据。更多还原

【Abstract】 Objective:The Box-Behnken experimental design effect surface method was applied in optimizing the production of adenosine by bi-directional fermentation of Ganoderma lucidum and Croton tiglium, which the antioxidant activity of Lingba Junzhi was also investigated. Methods:The factors that significantly affect the production of adenosine were selected by the single factor experiment and the Plackett-Burman experiment. Furthermore, the interaction among the initial water content, the inoculation volume, the fermentation time and the initial fermentation pH in the fermentation of Lingba Junzhi were investigated and optimized by Box-Behnken experimental design which the content of adenosine was used as an index. The antioxidant ability of Lingba Junzhi was evaluated based on its reducing power, DPPH radical scavenging ability and hydroxyl radical scavenging ability. Results:The results showed that the adenosine was 2.07 mg/g under initial water content of 80%, inoculation volume was 0.25 mg/g, fermentation time of 27 d, initial fermentation pH of 5. The antioxidant activity indicated that both the aqueous and alcoholic extracts of Lingba Junzhi had different degrees of reducing power and scavenging effect on DPPH radical and hydroxyl radical, with the aqueous extract being slightly more active.The antioxidant capacity of both was greater than that of the aqueous and alcoholic extracts of C. tiglium. Conclusion:The model developed in this study could be used to analyze and predict the adenosine content in the Lingba Junzhi and provided a basis for developing and utilizing C. tiglium resources.更多还原