【摘要】 目的 研究亚慢性摄入高剂量铁皮石斛对大鼠体重、进食量、食物利用率和血生化指标的影响，为评价铁皮石斛食用安全性提供依据。方法 80只SPF级SD大鼠随机分配入3个剂量组和1个对照组，每组20只，雌雄各半。低、中、高剂量组分别喂饲铁皮石斛饲料2.0、4.0和8.0 g/kg体重，对照组喂饲基础饲料，连续13周；观察并比较各组大鼠体重、进食量、食物利用率和血生化指标变化。结果 实验期间各组大鼠饮食、活动正常，未见异常症状和体征，无大鼠死亡。各组大鼠体重、进食量、总增重、总进食量、谷丙转氨酶、谷草转氨酶、尿素氮、肌酐、三酰甘油、胆固醇、总蛋白、白蛋白、白球比和血糖比较，差异均无统计学意义（P>0.05）。高剂量组雄性大鼠后7周食物利用率（8.71%±0.78%）和总食物利用率（18.00%±0.41%）均低于对照组（10.54%±1.37%、19.51%±1.21%）(P<0.05)。结论未发现亚慢性摄入高剂量铁皮石斛对大鼠的毒性作用，雄性大鼠食物利用率降低可能与铁皮石斛的保健功能有关。更多还原

【Abstract】 Objective To assess the effects of subchronic intake of high-dose Dendrobium officinale on body weight,food intake, food utilization, and blood biochemical parameters in rats, so as to provide insights into assessment of edible safety of D. officinale. Methods Eighty SPF-grade SD rats were randomly divided into the low-, medium-and high-dose groups and the control group, of 10 male and 10 female rats in each group. Rats in the low-, medium-and high-dose groups were administered with D. officinale feeds at doses of 2.0, 4.0, and 8.0 g/kg body weight, respectively, while animals in the control group were given basic diet for successive 13 weeks. The rat body weight, food intake,food utilization, and blood biochemical parameters were compared between groups. Results Normal diet and activity was seen in all rats, and no abnormal syndromes, signs or deaths were found during the study. There were no significant differences in rat body weight, food intake, total weight gain, total food intake, alanine aminotransferase, aspartate aminotransferase, urea nitrogen, creatinine, triacylglycerol, cholesterol, total protein, albumin, albumin/globulin ratio or blood glucose among four groups(P>0.05). The food utilization 7 weeks post-administration [(8.71% ± 0.78%) vs.(10.54%±1.37%), P<0.05] and the total food utilization [(18.00%±0.41%) vs.(19.51%±1.21%), P<0.05] were significantly lower in male rats in the high-dose group than in the control group. Conclusion Subchronic intake of high-dose D. officinale shows no toxicity in rats, and reduced food utilization may be associated with the health function of D. officinale in male rats.更多还原