【摘要】 烯醇化酶是参与代谢的关键酶,能够影响植物的生长发育和应答非生物胁迫。本研究克隆到一个玉米烯醇化酶基因ZmENO1,与高粱XP＿021305192.1 (SbENO1)亲缘性较高。ZmENO1受到干旱胁迫的诱导表达,且表达量与材料的耐旱性呈正相关。ZmENO1的过表达通过ABA依赖路径稳定细胞膜渗透势及增强抗氧化酶活性,从而提高了拟南芥对干旱胁迫的耐受力。有趣的是,发现ZmENO1可能是通过非依赖脱落酸(ABA)路径提高了过表达拟南芥株系的耐高温能力。酵母双杂实验表明ZmENO1参与了二氧化碳固定、信号传导和甘油酯、糖代谢等过程。本研究阐明了ZmENO1在拟南芥中响应干旱和高温胁迫的功能,并初步预测了其潜在的互作网络,为进一步解析ZmENO1的作用机制奠定了基础。更多还原

【Abstract】 Enolase is a key enzyme involved in metabolism, growth, development of plants and responds to abiotic stresses. In this study, ZmENO1, a maize enolase gene was cloned, which protein sequence was highly similarity with sorghum XP＿021305192.1(SbENO1). The expression of ZmENO1 was induced by drought stress, and the expression level was positively correlated with the drought tolerance of the material.Overexpression of ZmENO1 enhanced the tolerance of Arabidopsis to drought stress by stabilizing cell membrane osmotic potential and enhancing antioxidant enzyme activity through an ABA-dependent pathway. Interestingly, it was found that ZmENO1 enhanced the high temperature tolerance of overexpressing Arabidopsis lines possibly through an ABA-independent pathway. Yeast two-hybrid experiments showed that ZmENO1 was involved in carbon dioxide fixation, signal transduction and glyceride and sugar metabolism. This study clarified the function of ZmENO1 in response to drought and high temperature stress in Arabidopsis, and preliminarily predicted its potential interaction network, laying a foundation for further analysis of the mechanism of ZmENO1.更多还原