【摘要】 目的 分析缺氧诱导因子1α（HIF-1α）、25-羟维生素D₃[25-（OH）D₃]与支气管哮喘患儿疾病严重程度的关系。方法 将2020年2月—2022年6月在本院呼吸科住院治疗的127例支气管哮喘患儿按病情严重程度分轻度组（n=41）、中度组（n=58）、重度组（n=31）,另选取同期在本院接受健康体检的30例健康儿童作为对照组；比较各组HIF-1α、25-（OH）D₃水平及其他临床指标水平，多元逐步回归分析外周血HIF-1α、25-（OH）D₃与患儿病情严重程度的关系。结果 与对照组比较，轻度组、中度组、重度组HIF-1α均显著升高，25-（OH）D₃显著下降，且随着病情严重程度增加HIF-1α显著上升，重度组25-（OH）D₃显著低于轻度、中度组（均P<0.05）;与对照组比较，轻度组、中度组、重度组EFL指数、TNF-α、IL-6显著上升，H₂S显著下降，且随着病情严重程度增加，EFL、TNF-α、IL-6逐渐上升，H₂S逐渐下降（均P<0.05）;多元逐步回归分析，结果显示EFL指数、HIF-1α、25-OH-D₃、H₂S、TNF-α、IL-6均与哮喘患儿存在显著相关，均方差膨胀因子（VIF）<3。结论 哮喘患儿外周血HIF-1α、25-（OH）D₃与病情严重程度存在显著相关性，可作为评价哮喘患儿病情的潜在生物标志物。更多还原

【Abstract】 Objective This paper aims to analyze the relationship between hypoxia-inducible factor 1α（HIF-1α）, 25-hydroxyvitamin D₃[25-（OH）D₃] and the severity of bronchial asthma in children. Methods According to the severity of the disease, 127 children with bronchial asthma hospitalized in respiratory department of the hospital from February 2020 to June 2022 were divided into mild group（n=41）, moderate group（n=58）, and severe group（n=31）. Meanwhile, 30 healthy children were selected as the control group. The levels of HIF-1α, 25-（OH）D₃ and other clinical indicators were compared between the groups. The relationship between peripheral blood HIF-1α, 25-（OH）D₃ and the severity of bronchial asthma in children was analyzed by multiple stepwise regression method. Results Compared with the control group, the mild group, moderate group, and severe group had higher HIF-1α levels and lower 25-（OH）D₃ levels. As the condition aggravated, the HIF-1α level increased significantly. The 25-（OH）D₃ level in severe group was lower than that in the mild group and that in the moderate group（P<0.05）. Compared with the control group, the mild group, moderate group, and severe group had higher EFL index, TNF-α and IL-6 levels, and lower H₂S level. As the condition aggravated, EFL, TNF-α and IL-6 increased, and H₂S decreased（P<0.05）. Multiple stepwise regression analysis showed that EFL index, HIF-1α, 25-OH-D₃, H₂S, TNF-α, and IL-6 were correlated with the severity of asthma, and the mean variance inflation factor（VIF） was lower than 3. Conclusion There is a significant correlation between peripheral blood HIF-1α, 25-（OH）D₃ and the severity of bronchial asthma in children, which indicates that they can be used as potential biomarkers for evaluating the condition of children with asthma.更多还原