【摘要】 为研制新型绿色高效稻瘟病防控药剂，分别测定黄芩苷对稻瘟病菌Magnaporthe oryzae附着胞形成、菌落生长、菌丝侵染和致病力的影响以及对稻瘟病的防效。结果显示，黄芩苷抑制稻瘟病菌附着胞形成和菌落生长的最低浓度分别为100μmol/L和500μmol/L，在100μmol/L浓度下黄芩苷能抑制稻瘟病菌侵染菌丝在水稻叶鞘细胞中的扩展及叶片病斑的形成；在盆栽接种试验中，对照组的水稻苗表现为感病和中抗，所占比例分别为38%和62%，而黄芩苷处理组的水稻苗表现为抗病和中抗，其中表现为抗病的水稻苗比例超过70%；黄芩苷处理后，稻瘟病菌分生孢子细胞死亡率比对照组极显著降低。表明黄芩苷通过抑制稻瘟病菌分生孢子的细胞程序性死亡干扰了稻瘟病菌附着胞的形成，从而能有效防控稻瘟病，具有开发为新型绿色高效稻瘟病防控药剂的潜力。更多还原

【Abstract】 In order to develop a new green and efficient control agent on rice blast,the effects of baicalin on Magnaporthe oryzae appressorium formation,colony growth,hyphal infection,the pathogenicity to rice and the control efficacy on rice blast were tested.The results showed that the lowest concentration of baicalin inhibiting appressorium formation and colony growth in M.oryzae were 100 μmol/L and 500 μmol/L,respectively.At the concentration of 100 μmol/L baicalin,the expansion of M.oryzae infection hypha in rice leaf sheath cells and the formation of lesion on rice leaves were inhibited.In pot inoculation,the resistance levels of control rice seedlings(CK) were susceptible and moderately resistant,with the proportions of 38% and 62% respectively,while the resistance levels of rice seedlings with baicalin treatment group were resistant or moderately resistant,more than 70% seedlings showed resistance.Futhermore,the conidial cell death rate with the treatment of baicalin was significantly lower than the control group.The study showed that baicalin interfered with the appressorium formation of M.oryzae by inhibiting conidial programmed cell death,and significantly controlled rice blast.Therefore,baicalin has the potential to be developed as a novel green and efficient control agent on rice blast.更多还原