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HPLC法同时测定复方磺胺间甲氧嘧啶注射液中磺胺间甲氧嘧啶钠和甲氧苄啶含量

Content determination of sulfamonomethoxine sodium and trimethoprim in Compound Sulfamethoxine Sodium injection by HPLC

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【作者】 许世富潘孝成刘红云吴蕾许晓靖

【Author】 XU Shifu;PAN Xiaocheng;LIU Hongyun;WU Lei;XU Xiaojing;Anhui Provincial Control Institute of Veterinary Pharmaceuticals and Feed;Anhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering;

【通讯作者】 许世富;

【机构】 安徽省兽药饲料监察所畜禽产品安全工程安徽省重点实验室

【摘要】 利用高效液相色谱法(HPLC)同时测定复方磺胺间甲氧嘧啶钠注射液中磺胺间甲氧嘧啶钠和甲氧苄啶的含量。采用C18柱(5μm, 4.6 mm×250.0 mm),以乙腈∶0.1%磷酸溶液(20∶80,V/V)为流动相,检测波长230 nm,柱温30℃,流速1.0 mL/min。结果表明,磺胺间甲氧嘧啶在5.00~200.00μg/mL浓度范围内与峰面积线性关系良好(R2=0.999 6),平均回收率为99.49%,RSD为0.18%;甲氧苄啶在1.00~50.00μg/mL浓度范围内与峰面积线性关系良好(R2=0.999 7),平均回收率为99.02%,RSD为0.60%。本方法简单快捷,精密度好,可用于控制复方磺胺间甲氧嘧啶钠注射液中磺胺间甲氧嘧啶钠和甲氧苄啶的含量。

【Abstract】 The content sulfamethoxine sodium and trimethoprim in Compound Sulfamethoxine Sodium injection was determined by HPLC. Using C18 column(4.6 mm×250.0 mm, 5 μm) at 30℃, acetonitrile-0.1% phosphoric acid(20∶80, V∶V) was used as mobile phase, the detection wavelength was 230 nm, the flow rate was 1.0 mL·min-1. The results showed that sulfamethoxine had a good linear relationship with peak area in the range of 5.00-200.00 μg·mL-1(R2=0.999 6), the average recovery was 99.49% and RSD was 0.18%; trimethoprim had a good linear relationship with peak area in the range of 1.00-50.00 μg·mL-1(R2=0.999 7), the average recovery was 99.02% and RSD was 0.60%. The method is simple, rapid, accurate and can be used to control the contents of sulfamethoxine sodium and trimethoprim in Compound Sulfamethoxine Sodium injection.

【基金】 安徽省重点研究与开发计划(202204c06020009);安徽省现代农业产业技术体系(AHCYJXTX-05-13)
  • 【文献出处】 中兽医医药杂志 ,Journal of Traditional Chinese Veterinary Medicine , 编辑部邮箱 ,2023年01期
  • 【分类号】O657.72;S859.79
  • 【下载频次】29
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