【摘要】 目的 探讨丹参素通过经典Wnt/β-catenin信号通路改善滋养细胞氧化损伤中的作用机制。方法 将人绒毛滋养细胞系HTR-8/SVneo置于缺氧/复氧（hypoxia/reoxygenation, H/R）条件下，通过CCK-8以确定丹参素对细胞活力的影响以及后续实验的最佳处理浓度。设置正常对照组（normal control, NC）、正常培养+丹参素处理组（normal culture condition with danshensu treatment, NC+DS）、缺氧/复氧处理组（H/R condition, HR）和缺氧/复氧+丹参素处理组（H/R condition with danshensu treatment, HR+DS）。检测各组活性氧物质（reactive oxygen species, ROS）的水平差异。Western blot技术检测各组Wnt/β-catenin信号通路蛋白表达水平。结果 根据CCK-8的检测结果，丹参素最佳处理浓度为10μmol·L^-1,处理48 h后可显著恢复细胞活力。HTR-8/SVneo在H/R处理后ROS积累增加，丹参素处理后ROS积累显著减少。Western blot显示，H/R处理后HR组DKK1明显升高，而Wnt1、GSK-3β和β-catenin水平明显降低。经丹参素处理后HR+DS组Wnt1和β-catenin明显恢复。结论 研究结果表明，氧化应激抑制了Wnt1和β-catenin水平，抑制了Wnt/β-catenin信号通路，从而损害滋养细胞功能，促进滋养细胞凋亡，降低滋养细胞侵袭性。丹参素能减轻滋养细胞氧化应激，恢复Wnt1和β-catenin水平，重新激活Wnt/β-catenin信号通路。更多还原

【Abstract】 Objective To investigate the mechanism by which Danshensu enhances the protection against oxidative damage in trophoblast cells through activation of the classical Wnt/β-catenin signaling pathway.Methods The human chorionic trophoblast cell line HTR-8/SVneo was exposed to hypoxia/reoxygenation（H/R） conditions and subsequently assessed using CCK-8 assay to determine the impact of danshensu on cell viability and identify the optimal concentration for subsequent experiments.The experimental groups included a normal control group（NC）,a normal culture conditions with danshensu treatment group（NC+DS）,a H/R condition group（HR condition）,and a H/R condition with danshensu treatment group（HR+DS）.The levels of reactive oxygen species（ROS） exhibited intergroup variations, while the protein expression levels of the Wnt/β-catenin signaling pathway were evaluated through Western blot analysis.Results According to the CCK-8 results, danshensu exhibited an optimal treatment concentration of 10 μmol·L^-1,which significantly restored cell viability after 48 hours of treatment.The findings demonstrated an elevation in ROS accumulation in HTR-8/SVneo cells following H/R,which was subsequently significantly attenuated upon treatment with danshensu.Western blot showed that DKK1 was significantly elevated in the HR group after H/R treatment, while Wnt1,GSK-3β and β-catenin levels were significantly reduced.Wnt1 and β-catenin were significantly restored in the HR+DS group after danshensu treatment.Conclusion The results showed that oxidative stress exerted inhibitory effects on the levels1 and β-catenin, thereby suppressing the signaling pathway of Wnt/β-catenin, which subsequently compromised trophoblast function, facilitated trophoblast apoptosis, and diminished trophoblast invasiveness.Danshensu exhibited a mitigating effect on oxidative stress in trophoblasts, leading to the restoration of Wnt1 and β-catenin levels as well as reactivation of the Wnt/β-catenin signaling pathway.更多还原