【摘要】 为了确诊猪场1例疑似猪传染性胸膜肺炎的病例，本试验首先对该猪死亡前的临床症状进行了解，然后对死亡猪进行剖检并利用收集的病料进行细菌分离培养、质谱分析、PCR检测和组织病理学等实验室诊断。结果显示，该猪死亡前表现出体温升高、呼吸窘迫和鼻内流出血色样泡沫分泌物等临床症状，剖检发现胸腔积液、肺脏出血和肿大等病理变化。实验室诊断结果显示，胸腔积液、血液和肺脏等病料中分离获得的菌株为血清型7型胸膜肺炎放线杆菌。组织病理学观察结果显示，肺脏组织表现为出血性纤维素性肺炎，肺泡渗出液中可见蓝染的菌团；心脏、肾脏、淋巴结、肝脏和脾脏等其他脏器均有不同程度的出血、淤血、水肿和淋巴细胞浸润等组织病理学变化。综合以上诊断结果，确诊该猪为猪胸膜肺炎放线杆菌感染。更多还原

【Abstract】 In order to diagnose a suspected case of porcine contagious pleuropneumonia in a pig farm, this study first investigated the clinical symptoms before the pig’s death. Subsequently, a post-mortem examination was conducted, and laboratory diagnostics such as bacterial isolation culture, mass spectrometry analysis, PCR detection, and histopathology were performed on collected samples. The results revealed clinical symptoms including elevated body temperature, respiratory distress, and nasal discharge of bloody frothy secretions before the pig’s death. Post-mortem examination showed pathological changes such as pleural effusion, lung hemorrhage, and enlargement. Laboratory diagnostics confirmed the isolation of Actinobacillus pleuropneumoniae serotype 7 from pleural effusion, blood, and lung tissues. Histopathological observations demonstrated hemorrhagic fibrinous pneumonia in lung tissues with blue-stained bacterial clusters in alveolar exudate. Other organs such as the heart, kidneys, lymph nodes, liver, and spleen exhibited varying degrees of hemorrhage, congestion, edema, and lymphocytic infiltration. Based on the comprehensive diagnostic results, the pig was confirmed to be infected with Actinobacillus pleuropneumoniae.更多还原