【摘要】 为了解广西地区水牛感染中肠道病毒（BEV）的情况，本研究在广西南宁某水牛规模养殖场腹泻粪便样品中成功分离出2株BEV,分别命名为GXNN2106和GXNN2102。将2株病毒空斑纯化后测定GXNN2106毒株TCID₅₀为10^7.99/0.1 mL,GXNN2102毒株TCID₅₀为10^7.37/0.1 mL。多步生长曲线结果显示，GXNN2106毒株在MDBK细胞中复制增殖良好。经BEV VP1、5′UTR特异性引物逆转录聚合酶链反应（RT-PCR）鉴定，2株病毒均属于E2型，与其他E2型BEV 5′UTR核苷酸同源性为75.7%～78.6%,VP1核苷酸同源性为87.1%～91.1%。本研究首次在广西地区水牛粪便中分离得到E2型BEV毒株，为广西水牛源牛肠道病毒的流行分布情况和疫苗的研发提供数据支持。更多还原

【Abstract】 To investigate bovine enterovirus（BEV） infection status in buffalo of Guangxi, this study identified two BEV strains（GXNN2106 and GXNN2102） from the diarrheal fecal samples of a buffalo farm in Nanning, Guangxi, and determined the TCID₅₀ of GXNN2106 and GXNN2102 as 10^7.99/0.1 mL and 10^7.37/0.1 mL, respectively. The results of multi-step growth curve showed that GXNN2106 replicated well in MDBK cells. The two strains were identified as BEV E2 type by RT-PCR with BEV VP1 and 5’UTR specific primers; the nucleotide homology with other BEV E2 types 5’UTR was 75.7%-78.6%; and the nucleotide homology of VP1 was 87.1%-91.1%. Isolation of BEV E2 type strain from buffalo feces in Guangxi for the first time in this study facilitates further investigation on epidemiological distribution of BEV from buffalo in Guangxi as well as vaccine development.更多还原