【摘要】 移栽苗带菌是辣椒疫病发生面积不断增大的重要因素，为有效利用生防菌防控育苗期辣椒疫病，通过生防菌浸种法穴盘育苗检测了贝莱斯芽胞杆菌Bacillus velezensis LYK10和蜡样芽胞杆菌Bacillus cereus QTK2浸种对辣椒幼苗疫病的防治效果，并研究了生防菌混合使用浸种和菌药联用浸种的增效作用。结果表明，LYK10与QTK2单独浸种可延迟辣椒幼苗疫病的发生时间，防效分别为30.55%,33.33%，出苗率提高1.39%和2.78%。LYK10与QTK2可对疫霉菌产生拮抗作用，还可诱导辣椒植株CAT、PPO酶活变化。LYK10与QTK2等浓度混合浸种后10⁸、10⁷ cfu/m L混合菌液防效较两种单菌处理相比防效提高27.78%～42.45%。贝莱斯芽胞杆菌LYK10和烯酰吗啉混合浸种后，5.0×10⁷ cfu/m L+300μg/mL菌药联用处理防效为48.15%，高于10⁸ cfu/m L LYK10处理和300μg/mL烯酰吗啉处理，低浓度菌药联用处理防效为38.89%，高于LYK10处理而与烯酰吗啉处理相当。试验结果可为辣椒疫病生物防治和生防菌有效利用研究提供数据支撑。更多还原

【Abstract】 Transplanting seedlings with pathogen results in the spread of pepper blight.In order to effectively use biocontrol bacterium to prevent and control pepper blight at seedling stage,the efficacy of Bacillus velezensis LYK10 and Bacillus cereus QTK2 against pepper blight of seedling by soaking seed were tested by point plate seedling with biocontrol bacteria.The synergies of mixed biocontrol bacterium and biocontrol bacteria combined chemical fungicide were mensurated.The results showed that LYK10 and QTK2 could delay the occurrence time of pepper seedling disease,and the control efficiency of pepper seedling disease was respectively 30.55%and 33.33%,and the emergence rate was increased by 1.39%and 2.78%in turn.The both bacterium had antagonistic effects on Phytophthora capsici.,and also induced the changes of CAT and PPO enzyme activity in pepper plants.The efficiency of the two mixed bacterium at equal concentration improved from 27.78%to 42.45%compared with the two single bacteria treatments.The efficiency of the mixture of LYK10 and dienomorph with the consistence of 5.0×10⁷ cfu/mL+300μg/mL and 5.0×10⁶ cfu/mL+150μg/mL respectively were 48.15%and 38.89%.The control effect of high concentration bacterial-drug treatment was higher than the treatments of 10⁸ cfu/mL LYK10 and 300g/mL dienomorph.The control effect of low concentration bacterial-drug treatment was higher than LYK10 and comparable to dienomorph.The results can provide data to support for the research on biological control to pepper blight and effective utilization of biocontrol bacterium.更多还原