【摘要】 目的 了解辽宁省副溶血性弧菌的分布规律及流行趋势，为辽宁省副溶血性弧菌引起的食源性疾病的溯源和预警提供基础。方法 对2019年辽宁省151株VP分离株进行血清分型、PFGE、REP-PCR和ERIC-PCR分子分型和聚类分析，评价3种方法间的关联性，同时探讨菌株间的亲缘关系。结果 124株分离株可共分为18个血清型，27株分离株未能分型，血清群以O3群、O1群和O2群为主。PFGE的分辨力（DI）为0.97,REP-PCR的DI为0.95,ERIC-PCR的DI为0.93。血清型O3群菌株与O1群菌株分子型相似度高。结论 2019年辽宁省临床VP分离株流行血清型仍为O3:K6，食品VP分离株流行血清群仍为O2。PFGE、REP-PCR和ERIC-PCR 3种分型方法结果一致，且PFGE分型方法的分辨力和再现性均优于其他两种方法，研究结果可以为副溶血性弧菌所引起食源性疾病的溯源提供新的技术手段。更多还原

【Abstract】 Objective To provide a basis for the tracing and early warning of foodborne diseases caused by Vibrio parahaemolyticus(VP) in Liaoning Province, the distribution and epidemic trend of VP in Liaoning Province combined with serotype analysis were elucidated.Methods One hundred and fifty-one VP isolates from Liaoning Province in 2019 were serotyped, and PFGE, REP-PCR, and ERIC-PCR molecular typing and cluster analysis were performed to evaluate the correlation between the three methods and explore the kinship between the strains.Results One hundred and twenty-four isolates were divided into 18 serotypes and 27 isolates could not be classified. The main serotypes were the O3, O1, and O2 groups. The resolutions(DI) of PFGE, REP-PCR, and ERIC-PCR were 0. 97, 0. 95, and 0. 93, respectively. There was a higher similarity in the molecular type between the serotype O3 group strain and the O1 group strain.Conclusion The epidemic serogroup of clinical VP isolates in Liaoning Province is still O3:K6, and the epidemic serogroup of food VP isolates is still O2. The cluster analysis results of PFGE, REP-PCR, and ERIC-PCR are consistent, and the resolution and reproducibility of PFGE are better than those of the other two methods. These results can provide novel technical means for the traceability of foodborne diseases caused by VP.更多还原