【摘要】 目的 建立高胆碱饮食小鼠模型，探究高胆碱饮食对小鼠血清氨基酸代谢的影响。方法 选取20只SPF级4周龄小鼠随机分为2组：对照组给予普通鼠粮及实验室饮用水，高胆碱饮食组小鼠给予3%胆碱水溶液代替日常饮用水，饲养8周后采集小鼠血清，应用超高效液相色谱串联三重四极杆质谱仪（UPLC-MS/MS）定量检测血清氨基酸水平。采用主成分分析和正交偏最小二乘-判别分析判断高胆碱饮食组和对照组氨基酸组成相似性。结果 高胆碱饮食组与对照组相比，胱氨酸、丝氨酸、丙氨酸、谷氨酸、苯丙氨酸、天冬酰胺、苏氨酸、脯氨酸、反式4-羟基脯氨酸和支链氨基酸水平显著升高，赖氨酸、瓜氨酸水平显著降低，组间比较差异具有统计学意义（P<0.05）。支链氨基酸中异亮氨酸水平由34.20±2.71升高至72.59±10.42μmol/L；亮氨酸水平由68.59±4.06升高至113.04±14.64μmol/L；缬氨酸水平由129.86±10.54升高至161.95±16.54μmol/L。结论 高胆碱饮食引起小鼠血清氨基酸代谢发生显著变化，其中精氨酸、脯氨酸、异亮氨酸、亮氨酸、缬氨酸、苯丙氨酸、组氨酸、赖氨酸可作为高胆碱饮食引起氨基酸代谢异常的生物标志物。更多还原

【Abstract】 Objective To establish a high choline diet mouse model and investigate the effect of high choline diet on serum amino acid metabolism in mice.Methods Twenty SPF-grade 4-week-old mice were randomly divided into two groups, the control group was given ordinary mouse food and laboratory drinking water, and the high-choline diet group were given 3% choline aqueous solution instead of daily drinking water. Serum was collected after 8 weeks of feeding, and serum amino acids levels were quantified by ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry(UPLC-MS/MS). Principal component analysis and orthogonal partial least squares-discriminant analysis were used to determine the similarity of amino acid composition between the high choline diet group and the control group.Results Compared with the control group, the levels of cystine, serine, alanine, glutamic acid, phenylalanine, asparagine, threonine, proline, trans-4-hydroxyproline and branched-chain amino acids were significantly higher and the levels of lysine were significantly lower in the high-choline diet group, with isoleucine levels of 34. 20±2. 71 and 72. 59±10. 42 μmol/L; the levels of leucine were 68. 59±4. 06 and 113. 04±14. 64 μmol/L; the levels of valine were 129. 86±10. 54 and 161. 95±16. 54 μmol/L, respectively, and the differences between groups were statistically significant(P<0. 05).Conclusion The high choline diet cause significant changes in serum amino acid metabolism in mice.更多还原