【摘要】 目的 为迅速精准鉴定新型SARS-CoV-2变异株，采用“二代+三代”联用技术对福建省2例输入性SARS-CoV-2感染病例标本进行全基因组测序和变异分析。方法 分别采用Nanopore和Illumina技术进行病毒全基因组测序，应用Pangolin系统判定病毒型别，利用进化分析软件构建系统发育树，使用Nextclade进行病毒全基因组突变分析、ACE2受体亲和力及免疫逃逸能力评估。结果 自2例病例呼吸道标本中获得2株SARS-CoV-2全基因组序列，长度分别为29 665 bp和29 682 bp,基因组平均覆盖度>99.0%,均为Omicron变异株BQ.1进化分支；进化分析表明2株病毒在BQ.1进化分支同一簇上，结合流调资料，推测2个病例可能来自同一传染源；突变分析显示2株病毒共享全部77个核苷酸突变位点，除S:A27S和S:24-26del外，其余均为BQ.1变异株的特征性突变；2株病毒ACE2受体亲和力及免疫逃逸能力的预测分值均>0.6,推测其生物学特性发生较明显变化，需给予持续监测预警。结论 成功应用“二代+三代”测序技术证实福建省首发输入性BQ.1进化分支感染病例，该技术兼顾测序的准确性和时效性，可为SARS-CoV-2变异监测提供技术参考。更多还原

【Abstract】 To rapidly and accurately identify novel SARS-CoV-2 variants, we used combined 2nd and 3rd generation sequencing technology to sequence and analyze the viral genomes of two specimens from SARS-CoV-2 infection cases imported into Fujian Province.Nanopore and Illumina techniques were used to perform whole genome sequencing of SARS-CoV-2.A pangolin system was used to determine the virus type. Evolutionary analysis software was used to construct the phylogenetic tree.Nextclade was used to analyze the whole genome variation, and estimate ACE2 receptor affinity and immune escape ability.Two complete genome sequences of SARS-CoV-2 were obtained from respiratory specimens from the two cases, with lengths of 29 665 bp and 29 682 bp. The average genome coverage were>99.0%, and the virus typing results all indicated Omicron BQ.1 lineage. Phylogenetic analysis demonstrated that the two viruses were located in the same cluster of the Omicron BQ.1 lineage. On the basis of these findings and epidemiological data, we speculated that the two cases might have originated from the same infection. Variation analysis indicated that the two viruses shared all 77 mutation sites; except for S:A27S and S:24-26del, all were characteristic mutation sites of the BQ.1 lineage. The predicted ACE2 receptor affinity and immune escape ability scores of the two viruses were both>0.6, thus suggesting significant changes in their biological characteristics and requiring continuous monitoring and warning.The combined 2nd and 3rd generation sequencing technology was successfully applied to identify the first BQ.1 lineage of the SARS-CoV-2 imported into Fujian Province, considering the timeliness and accuracy of whole genome sequencing, thus providing a technical reference for SARS-CoV-2 variant monitoring.更多还原