【摘要】 目的 建立疏清颗粒高效液相色谱（HPLC）指纹图谱，为疏清颗粒标准提供参考。方法采用HPLC法，利用Inertsil-ODS-3色谱柱（4.6 mm×250 mm,5μm），以乙腈-0.3%磷酸溶液为流动相进行梯度洗脱，检测波长242 nm，流速1.0 mL·min^-1，柱温35℃，进样量为10μL。运用“中药色谱指纹图谱相似度评价系统（2004A版）”评价10批疏清颗粒指纹图谱的相似度。结果 10批疏清颗粒相似度大于0.98，标出了31个共有峰，指认出甘草苷、甘草酸、芦丁、阿魏酸、绿原酸5个成分。结论 该方法具有良好的稳定性和重复性，可用于疏清颗粒的质量评价。更多还原

【Abstract】 Objective To establish a high performance liquid chromatography（HPLC） fingerprint detection method for Shuqing granules and to provide reference for the standards of Shuqing granules.Methods HPLC was used. Inertsil-ODS-3 column（4.6 mm×250 mm, 5 μm） was used with a mobile phase of acetonitrile-0.3% phosphoric acid solution. The detection wavelength was 242 nm, the flow rate was 1.0 mL·min^-1, the column temperature was 35 ℃, and the injection volume was 10 μL.Similarity evaluation system of chromatographic fingerprint of traditional Chinese medicine（2004A Edition） was used to analyze the chromatographic data of 10 batches of Shuqing granules. Results The similarities of the 10 batches of samples were greater than 0.98, and 31 common peaks were marked. Five components including licorice side, glycyrrhizic acid, rutin, ferulic acid and chlorogenic acid were identified. Conclusion The method can be used for the quality evaluation of Shuqing granules owing to good great stability and reproducibility.更多还原