【摘要】 为了优化北苍术中苍术素、苍术酮、β-桉叶醇的提取过程及HPLC测定方法。分别对北苍术用量、超声时间、超声温度做了测试，并对色谱条件逐一考察。确定提取条件：北苍术0.8 g,40℃超声60 min。色谱条件：色谱柱(Venusil MP C18 4.6*150 mm,5μm,150 A)，柱温28℃。以甲醇:水(82:18)为流动相检测苍术素、苍术酮。以乙腈：0.2%碳酸氢钠：10%甲醇(65:25:10)为流动相检测β-桉叶醇。在0.002～1 mg/mL范围内，苍术素、苍术酮、β-桉叶醇峰面积线性关系良好(R~2=0.9999～1,n=6)。平均加样回收率为97.7%～102.17%,RSD为1.52%～2.49%,n=6。该方法准确、简便，可用于1～4年生北苍术中苍术素、苍术酮、β-桉叶醇的含量检测。更多还原

【Abstract】 To optimize the extraction procedure of atractylodin, atractylone and β-Eudesmol of Atractylodes chinensis(DC.) Koidz and the HPLC analytical method, the sample consumption, ultrasonic time and ultrasonic temperature in the procedure of sample extraction were analyzed, and chromatographic conditions were investigated one by one. The extraction condition was as follows: sample consumption of 0.8 g, and ultrasonication at 40℃ for 60 min. The chromatographic condition was as follows: chromatographic column(Venusil MP C18, 4.6×150 mm, 5 μm, 150 A), and column temperature of 28℃. To detect atractylodin and atractylone, methanol: water(82:18) was used as the mobile phase; to detect β-Eudesmol, acetonitrile: 0.2%sodium bicarbonate: 10% methanol(65:25:10) was used as the mobile phase. In the range of 0.002-1 mg/mL,the peak areas of atractylodin, atractylone and β-Eudesmol had good linear relationships(R~2=0.9999-1, n=6).The average recovery ratio was 97.7%-102.17%, RSD was 1.52%-2.49%, n=6. This method is accurate and simple and it could be used for determining the content of atractylodin, atractylone and β-Eudesmol of A.chinensis of 1-4 years old.更多还原